dc.contributor.author | Eggestøl, Håvard Øritsland | |
dc.contributor.author | Haugland, Gyri Teien | |
dc.contributor.author | Lunde, Harald S. | |
dc.date.accessioned | 2021-03-25T10:23:13Z | |
dc.date.available | 2021-03-25T10:23:13Z | |
dc.date.created | 2020-01-10T06:21:23Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 0145-305X | |
dc.identifier.uri | https://hdl.handle.net/11250/2735471 | |
dc.description.abstract | The proinflammatory cytokines TNF-α and IL-6 are important mediators of inflammatory reactions and orchestrators of the immune system in vertebrate. In this study, we have identified TNF-α and IL-6 in lumpfish, molecular characterized them at mRNA and gene level, performed homology modelling and measured their gene expression in different tissues and upon in vitro stimulation. A comprehensive phylogenetic analysis of TNF-α teleost sequences give novel insight into the TNF –α biology. Interestingly, we identified two isoforms of luIL-6. In normal tissue and leukocyte, the level of luTNF-α transcripts was higher than luIL-6. The expression pattern were parallel, except for brain, eye and gonad, and they displayed a similar induction pattern upon exposure to PAMPs, being most highly upregulated by flagellin. This is the first in-depth characterization of TNF and IL-6 in lumpfish. In recent years, lumpfish has become an important species for the aquaculture industry and establishment of qPCR-assays of luTNF-α and luIL-6 provide a valuable tool to measure effect of immune modulation, such as vaccination, microbiological disease and physiological trials. Lumpfish is also interesting for comparative studies as it represent a phylogenetic group that is poorly described immunologically. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | The proinflammatory cytokines TNF-α and IL-6 in lumpfish (Cyclopterus lumpus L.) -identification, molecular characterization, phylogeny and gene expression analyses | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2020 The Authors. | en_US |
dc.source.articlenumber | 103608 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.doi | https://doi.org/10.1016/j.dci.2020.103608 | |
dc.identifier.cristin | 1769928 | |
dc.source.journal | Developmental and Comparative Immunology | en_US |
dc.source.40 | 106 | |
dc.source.14 | 103608 | |
dc.relation.project | Norges forskningsråd: 244148 | en_US |
dc.identifier.citation | Developmental and Comparative Immunology. 2020, 105, 103608. | en_US |
dc.source.volume | 105 | en_US |