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dc.contributor.authorFournel, Rémi
dc.contributor.authorHartveit, Espen
dc.contributor.authorVeruki, Margaret Lin
dc.PublishedCellular and molecular neurobiology. 2020, .
dc.description.abstractGap junctions are ubiquitous within the retina, but in general, it remains to be determined whether gap junction coupling between specific cell types is sufficiently strong to mediate functionally relevant coupling via electrical synapses. From ultrastructural, tracer coupling and immunolabeling studies, there is clear evidence for gap junctions between cone bipolar cells, but it is not known if these gap junctions function as electrical synapses. Here, using whole-cell voltage-clamp recording in rat (male and female) retinal slices, we investigated whether the gap junctions of bipolar cells make a measurable contribution to the membrane properties of these cells. We measured the input resistance (RN) of bipolar cells before and after applying meclofenamic acid (MFA) to block gap junctions. In the presence of MFA, RN of ON-cone bipolar cells displayed a clear increase, paralleled by block of the electrical coupling between these cells and AII amacrine cells in recordings of coupled cell pairs. For OFF-cone and rod bipolar cells, RN did not increase in the presence of MFA. The results for rod bipolar cells are consistent with the lack of gap junctions in these cells. However, for OFF-cone bipolar cells, our results suggest that the morphologically identified gap junctions between these cells do not support a junctional conductance that is sufficient to mediate effective electrical coupling. Instead, these junctions might play a role in chemical and/or metabolic coupling between subcellular compartments.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.subjectNeurovitenskap / nevrovitenskapen_US
dc.titleDifferential contribution of gap junctions to the membrane properties of ON- and OFF-bipolar cells of the rat retinaen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.rights.holderCopyright 2020 The Authorsen_US
dc.source.journalCellular and molecular neurobiologyen_US
dc.relation.projectNorges forskningsråd: 261914en_US
dc.relation.projectNorges forskningsråd: 182743en_US
dc.relation.projectNorges forskningsråd: 214216en_US
dc.relation.projectNorges forskningsråd: 189662en_US
dc.subject.nsiVDP::Basale biofag: 470en_US
dc.subject.nsiVDP::Basic biosciences: 470en_US
dc.identifier.citationCellular and molecular neurobiology. 2020, 41, 229–245en_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal