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dc.contributor.authorBalafkan, Novin
dc.contributor.authorMostafavi, Sepideh
dc.contributor.authorSchubert, Manja
dc.contributor.authorSiller, Richard
dc.contributor.authorLiang, Xiao
dc.contributor.authorSullivan, Gareth
dc.contributor.authorBindoff, Laurence
dc.date.accessioned2021-04-27T11:56:46Z
dc.date.available2021-04-27T11:56:46Z
dc.date.created2020-10-29T09:21:25Z
dc.date.issued2020
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/11250/2739916
dc.description.abstractThe capacity of pluripotent stem cells both for self-renewal and to differentiate into any cell type have made them a powerful tool for studying human disease. Protocols for efficient differentiation towards cardiomyocytes using defined, serum-free culture medium combined with small molecules have been developed, but thus far, limited to larger formats. We adapted protocols for differentiating human pluripotent stem cells to functional human cardiomyocytes in a 96-well microplate format. The resulting cardiomyocytes expressed cardiac specific markers at the transcriptional and protein levels and had the electrophysiological properties that confirmed the presence of functional cardiomyocytes. We suggest that this protocol provides an incremental improvement and one that reduces the impact of heterogeneity by increasing inter-experimental replicates. We believe that this technique will improve the applicability of these cells for use in developmental biology and mechanistic studies of disease.en_US
dc.language.isoengen_US
dc.publisherNature Researchen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleA method for dierentiating human induced pluripotent stem cells toward functional cardiomyocytes in 96‐well microplatesen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2020 The Author(s).en_US
dc.source.articlenumber18498en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.doihttps://doi.org/10.1038/s41598-020-73656-2
dc.identifier.cristin1843156
dc.source.journalScientific Reportsen_US
dc.identifier.citationScientific Reports. 2020, 10, 18498en_US
dc.source.volume10en_US


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal