dc.contributor.author | Mohn, Kristin Greve-Isdahl | |
dc.contributor.author | Brokstad, Karl Albert | |
dc.contributor.author | Islam, Shahinul | |
dc.contributor.author | Oftung, Fredrik | |
dc.contributor.author | Tøndel, Camilla | |
dc.contributor.author | Aarstad, Hans Jørgen | |
dc.contributor.author | Cox, Rebecca Jane | |
dc.date.accessioned | 2021-05-26T12:10:06Z | |
dc.date.available | 2021-05-26T12:10:06Z | |
dc.date.created | 2020-04-08T08:15:22Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 0022-1899 | |
dc.identifier.uri | https://hdl.handle.net/11250/2756455 | |
dc.description.abstract | Background
Live-attenuated influenza vaccine (LAIV) was licensed for prophylaxis of children 2–17 years old in Europe in 2012 and is administered as a nasal spray. Live-attenuated influenza vaccine induces both mucosal and systemic antibodies and systemic T-cell responses. Tonsils are the lymph nodes serving the upper respiratory tract, acting as both induction and effector site for mucosal immunity.
Methods
Here, we have studied the early tonsillar T-cell responses induced in children after LAIV. Thirty-nine children were immunized with trivalent LAIV (containing A/H1N1, A/H3N2, and B viruses) at days 3, 7, and 14 before tonsillectomy. Nonvaccinated controls were included for comparison. Tonsils and peripheral blood (pre- and postvaccination) were collected to study T-cell responses.
Results
Tonsillar and systemic T-cell responses differed between influenza strains, and both were found against H3N2 and B viruses, whereas only systemic responses were observed against A/H1N1. A significant increase in cross-reactive tonsillar CD8+ T cells recognizing conserved epitopes from a broad range of seasonal and pandemic viruses occurred at day 14. Tonsillar T cells showed significant cytokine responses (Th1, Th2, and granulocyte-macrophage colony-stimulating factor).
Conclusions
Our findings support the use of LAIV in children to elicit broadly cross-reactive T cells, which are not induced by traditional inactivated influenza vaccines and may provide protection to novel virus strains. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Oxford University Press | en_US |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no | * |
dc.title | Early Induction of Cross-Reactive CD8+ T-Cell Responses in Tonsils After Live-Attenuated Influenza Vaccination in Children | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2019 The Authors | en_US |
dc.source.articlenumber | 1528–1537 | en_US |
cristin.ispublished | true | |
cristin.fulltext | postprint | |
cristin.qualitycode | 2 | |
dc.identifier.doi | 10.1093/infdis/jiz583 | |
dc.identifier.cristin | 1805611 | |
dc.source.journal | Journal of Infectious Diseases | en_US |
dc.relation.project | Norges forskningsråd: 271160 | en_US |
dc.relation.project | Norges forskningsråd: 284930 | en_US |
dc.relation.project | Norges forskningsråd: 811752 | en_US |
dc.relation.project | Norges forskningsråd: 815818 | en_US |
dc.identifier.citation | Journal of Infectious Diseases. 2020, 221(9), 1528–1537 | en_US |
dc.source.volume | 221 | en_US |
dc.source.issue | 9 | en_US |