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dc.contributor.authorBeigl, Tobias B.
dc.contributor.authorKjosås, Ine
dc.contributor.authorSeljeseth, Emilie
dc.contributor.authorGlomnes, Nina
dc.contributor.authorAksnes, Henriette
dc.date.accessioned2021-07-07T11:36:36Z
dc.date.available2021-07-07T11:36:36Z
dc.date.created2021-02-15T11:14:40Z
dc.date.issued2020
dc.identifier.issn2046-6390
dc.identifier.urihttps://hdl.handle.net/11250/2763778
dc.description.abstractThe near-haploid human cell line HAP1 recently became a popular subject for CRISPR/Cas9 editing, since only one allele requires modification. Through the gene-editing service at Horizon Discovery, there are at present more than 7500 edited cell lines available and the number continuously increases. The haploid nature of HAP1 is unstable as cultures become diploid with time. Here, we demonstrated some fundamental differences between haploid and diploid HAP1 cells, hence underlining the need for taking control over ploidy status in HAP1 cultures prior to phenotyping. Consequently, we optimized a procedure to determine the ploidy of HAP1 by flow cytometry in order to obtain diploid cultures and avoid ploidy status as an interfering variable in experiments. Furthermore, in order to facilitate this quality control, we validated a size-based cell sorting procedure to obtain the diploid culture more rapidly. Hence, we provide here two streamlined protocols for quality controlling the ploidy of HAP1 cells and document their validity and necessity.en_US
dc.language.isoengen_US
dc.publisherThe Company of Biologistsen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleEfficient and crucial quality control of HAP1 cell ploidy statusen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2020. Published by The Company of Biologists Ltd.en_US
dc.source.articlenumberbio057174en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.doi10.1242/bio.057174
dc.identifier.cristin1889780
dc.source.journalBiology Openen_US
dc.relation.projectNorges forskningsråd: 249843en_US
dc.identifier.citationBiology Open. 2020, 9 (11), bio057174.en_US
dc.source.volume9en_US
dc.source.issue11en_US


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