dc.contributor.author | Lea, Dordi | |
dc.contributor.author | Watson, Martin Matthew Christian | |
dc.contributor.author | Skaland, Ivar | |
dc.contributor.author | Hagland, Hanne Røland | |
dc.contributor.author | Lillesand, Melinda | |
dc.contributor.author | Gudlaugsson, Einar | |
dc.contributor.author | Søreide, Kjetil | |
dc.date.accessioned | 2021-08-09T11:38:19Z | |
dc.date.available | 2021-08-09T11:38:19Z | |
dc.date.created | 2021-04-21T18:23:03Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 0340-7004 | |
dc.identifier.uri | https://hdl.handle.net/11250/2767011 | |
dc.description.abstract | Background: In colon cancer, the location and density of tumor-infiltrating lymphocytes (TILs) can classify patients into low and high-risk groups for prognostication. While a commercially available ‘Immunoscore®’ exists, the incurred expenses and copyrights may prevent universal use. The aim of this study was to develop a robust and objective quantification method of TILs in colon cancer.
Methods: A consecutive, unselected series of specimens from patients with colon cancer were available for immunohistochemistry and assessment of TILs by automated digital pathology. CD3 + and CD8 + cells at the invasive margin and in tumor center were assessed on consecutive sections using automated digital pathology and image analysis software (Visiopharm®). An algorithm template for whole slide assessment, generated cell counts per square millimeters (cells/mm2), from which the immune score was calculated using distribution volumes. Furthermore, immune score was compared with clinical and histopathological characteristics to confirm its relevance.
Results: Based on the quantified TILs numbers by digital image analyses, patients were classified into low (n = 83, 69.7%), intermediate (n = 14, 11.8%) and high (n = 22, 18.5%) immune score groups. High immune score was associated with stage I–II tumors (p = 0.017) and a higher prevalence of microsatellite instable (MSI) tumors (p = 0.030). MSI tumors had a significantly higher numbers of CD3 + TILs in the invasive margin and CD8 + TILs in both tumor center and invasive margin, compared to microsatellite stable (MSS) tumors.
Conclusion: A digital template to quantify an easy-to-use immune score corresponds with clinicopathological features and MSI in colon cancer. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Springer | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | A template to quantify the location and density of CD3 + and CD8 + tumor-infiltrating lymphocytes in colon cancer by digital pathology on whole slides for an objective, standardized immune score assessment | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2021 The Authors | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.doi | 10.1007/s00262-020-02834-y | |
dc.identifier.cristin | 1905703 | |
dc.source.journal | Cancer Immunology and Immunotherapy | en_US |
dc.source.pagenumber | 2049–2057 | en_US |
dc.identifier.citation | Cancer Immunology and Immunotherapy. 2021, 70, 2049-2057. | en_US |
dc.source.volume | 70 | en_US |