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dc.contributor.authorLea, Dordi
dc.contributor.authorWatson, Martin Matthew Christian
dc.contributor.authorSkaland, Ivar
dc.contributor.authorHagland, Hanne Røland
dc.contributor.authorLillesand, Melinda
dc.contributor.authorGudlaugsson, Einar
dc.contributor.authorSøreide, Kjetil
dc.date.accessioned2021-08-09T11:38:19Z
dc.date.available2021-08-09T11:38:19Z
dc.date.created2021-04-21T18:23:03Z
dc.date.issued2021
dc.identifier.issn0340-7004
dc.identifier.urihttps://hdl.handle.net/11250/2767011
dc.description.abstractBackground In colon cancer, the location and density of tumor-infiltrating lymphocytes (TILs) can classify patients into low and high-risk groups for prognostication. While a commercially available ‘Immunoscore®’ exists, the incurred expenses and copyrights may prevent universal use. The aim of this study was to develop a robust and objective quantification method of TILs in colon cancer. Methods A consecutive, unselected series of specimens from patients with colon cancer were available for immunohistochemistry and assessment of TILs by automated digital pathology. CD3 + and CD8 + cells at the invasive margin and in tumor center were assessed on consecutive sections using automated digital pathology and image analysis software (Visiopharm®). An algorithm template for whole slide assessment, generated cell counts per square millimeters (cells/mm2), from which the immune score was calculated using distribution volumes. Furthermore, immune score was compared with clinical and histopathological characteristics to confirm its relevance. Results Based on the quantified TILs numbers by digital image analyses, patients were classified into low (n = 83, 69.7%), intermediate (n = 14, 11.8%) and high (n = 22, 18.5%) immune score groups. High immune score was associated with stage I–II tumors (p = 0.017) and a higher prevalence of microsatellite instable (MSI) tumors (p = 0.030). MSI tumors had a significantly higher numbers of CD3 + TILs in the invasive margin and CD8 + TILs in both tumor center and invasive margin, compared to microsatellite stable (MSS) tumors. Conclusion A digital template to quantify an easy-to-use immune score corresponds with clinicopathological features and MSI in colon cancer.en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleA template to quantify the location and density of CD3 + and CD8 + tumor-infiltrating lymphocytes in colon cancer by digital pathology on whole slides for an objective, standardized immune score assessmenten_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2021 The Authorsen_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.doi10.1007/s00262-020-02834-y
dc.identifier.cristin1905703
dc.source.journalCancer Immunology and Immunotherapyen_US
dc.source.pagenumber2049–2057en_US
dc.identifier.citationCancer Immunology and Immunotherapy. 2021, 70, 2049-2057.en_US
dc.source.volume70en_US


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal