Vis enkel innførsel

dc.contributor.authorZhao, Lin
dc.contributor.authorSu, Jiali
dc.contributor.authorLiu, Sijia
dc.contributor.authorLi, Yang
dc.contributor.authorXi, Tao
dc.contributor.authorRuan, Jianping
dc.contributor.authorLiang, Xiao
dc.contributor.authorHuang, Ruizhe
dc.date.accessioned2021-08-10T14:15:54Z
dc.date.available2021-08-10T14:15:54Z
dc.date.created2021-04-26T14:24:45Z
dc.date.issued2021
dc.identifier.issn0006-291X
dc.identifier.urihttps://hdl.handle.net/11250/2767229
dc.descriptionUnder embargo until: 2022-01-22en_US
dc.description.abstractDental fluorosis is characterized by hypomineralization of tooth enamel caused by ingestion of excessive fluoride during enamel formation. Excess fluoride could have effects on the ERK signaling, which is essential for the ameloblasts differentiation and tooth development. MAP kinase phosphatase-1 (MKP-1) plays a critical role in regulating ERK related kinases. However, the role of MKP-1 in ameloblast and the mechanisms of MKP-1/ERK signaling in the pathogenesis of dental fluorosis are incompletely understood. Here, we adopted an in vitro fluorosis cell model using murine ameloblasts-like LS8 cells by employing sodium fluoride (NaF) as inducer. Using this system, we demonstrated that fluoride exposure led to an inhibition of p-MEK and p-ERK1/2 with a subsequent increase in MKP-1 expression in a dose-dependent manner. We further identified, under high dose fluoride, MKP-1 acted as a negative regulator of the fluoride-induced p-ERK1/2 signaling, leading to downregulation of CREB, c-myc, and Elk-1. Our results identify a novel MKP-1/ERK signaling mechanism that regulates dental fluorosis and provide a framework for studying the molecular mechanisms of intervention and fluorosis remodeling under normal and pathological conditions. MKP-1 inhibitors may prove to be a benefit therapeutic strategy for dental fluorosis treatment.en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.no*
dc.titleMAP kinase phosphatase MKP-1 regulates p-ERK1/2 signaling pathway with fluoride treatmenten_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionacceptedVersionen_US
dc.rights.holderCopyright 2021 Elsevieren_US
cristin.ispublishedtrue
cristin.fulltextpostprint
cristin.qualitycode1
dc.identifier.doi10.1016/j.bbrc.2020.12.100
dc.identifier.cristin1906451
dc.source.journalBiochemical and Biophysical Research Communications - BBRCen_US
dc.source.pagenumber65-72en_US
dc.identifier.citationBiochemical and Biophysical Research Communications - BBRC. 2021, 542, 65-72.en_US
dc.source.volume542en_US


Tilhørende fil(er)

Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel

Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
Med mindre annet er angitt, så er denne innførselen lisensiert som Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal