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dc.contributor.authorHoel, Ida Marie
dc.contributor.authorAli, Iman A Mohammed
dc.contributor.authorIshtiaq, Sheeba
dc.contributor.authorSviland, Lisbet
dc.contributor.authorWiker, Harald Gotten
dc.contributor.authorMustafa, Tehmina
dc.date.accessioned2021-11-19T07:54:16Z
dc.date.available2021-11-19T07:54:16Z
dc.date.created2021-09-10T10:34:24Z
dc.date.issued2021
dc.identifier.issn2073-4468
dc.identifier.urihttps://hdl.handle.net/11250/2830389
dc.description.abstractTuberculosis (TB) is a global health problem. The immunohistochemistry (IHC)-based MPT64 antigen detection test has shown promising results for diagnosing extrapulmonary TB in previous studies. However, the anti-MPT64 antibody currently used in the test is in limited supply, and reproduction of a functional antibody is a prerequisite for further large-scale use. Various antigen-adjuvant combinations and immunisation protocols were tested in mice and rabbits to generate monoclonal and polyclonal antibodies. Antibodies were screened in IHC, and the final new antibody was validated on clinical human specimens. We were not able to generate monoclonal antibodies that were functional in IHC, but we obtained multiple functional polyclonal antibodies through careful selection of antigen-adjuvant and comprehensive screening in IHC of both pre-immune sera and antisera. To overcome the limitation of batch-to-batch variability with polyclonal antibodies, the best performing individual polyclonal antibodies were pooled to one final large-volume new anti-MPT64 antibody. The sensitivity of the new antibody was in the same range as the reference antibody, while the specificity was somewhat reduced. Our results suggest that it possible to reproduce a large-volume functional polyclonal antibody with stable performance, thereby securing stable supplies and reproducibility of the MPT64 test, albeit further validation remains to be done.en_US
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleImmunochemistry-Based Diagnosis of Extrapulmonary Tuberculosis: A Strategy for Large-Scale Production of MPT64-Antibodies for Use in the MPT64 Antigen Detection Testen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2021 by the authorsen_US
dc.source.articlenumber34en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.doi10.3390/antib10030034
dc.identifier.cristin1933109
dc.source.journalAntibodiesen_US
dc.identifier.citationAntibodies. 2021, 10 (3), 34.en_US
dc.source.volume10en_US
dc.source.issue3en_US


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