Show simple item record

dc.contributor.authorValen, Eivind
dc.contributor.authorLabun, Kornel
dc.contributor.authorMontague, Tessa G.
dc.contributor.authorGagnon, James A.
dc.contributor.authorThyme, Summer B.
dc.date.accessioned2022-01-24T09:30:46Z
dc.date.available2022-01-24T09:30:46Z
dc.date.created2017-02-13T22:40:49Z
dc.date.issued2016
dc.identifier.issn0305-1048
dc.identifier.urihttps://hdl.handle.net/11250/2838876
dc.description.abstractIn just 3 years CRISPR genome editing has transformed biology, and its popularity and potency continue to grow. New CRISPR effectors and rules for locating optimum targets continue to be reported, highlighting the need for computational CRISPR targeting tools to compile these rules and facilitate target selection and design. CHOPCHOP is one of the most widely used web tools for CRISPR- and TALEN-based genome editing. Its overarching principle is to provide an intuitive and powerful tool that can serve both novice and experienced users. In this major update we introduce tools for the next generation of CRISPR advances, including Cpf1 and Cas9 nickases. We support a number of new features that improve the targeting power, usability and efficiency of CHOPCHOP. To increase targeting range and specificity we provide support for custom length sgRNAs, and we evaluate the sequence composition of the whole sgRNA and its surrounding region using models compiled from multiple large-scale studies. These and other new features, coupled with an updated interface for increased usability and support for a continually growing list of organisms, maintain CHOPCHOP as one of the leading tools for CRISPR genome editing. CHOPCHOP v2 can be found at http://chopchop.cbu.uib.no.en_US
dc.language.isoengen_US
dc.publisherOxford University Pressen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleCHOPCHOP v2: a web tool for the next generation of CRISPR genome engineeringen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2016 The Author(s)en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2
dc.identifier.doi10.1093/nar/gkw398
dc.identifier.cristin1450168
dc.source.journalNucleic Acids Research (NAR)en_US
dc.source.pagenumberW272–W276en_US
dc.relation.projectBergens forskningsstiftelse: -en_US
dc.identifier.citationNucleic Acids Research (NAR). 2016, 44, W272–W276en_US
dc.source.volume44en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal