dc.contributor.author | Islam, Tajul | |
dc.contributor.author | Hernández, Marcela | |
dc.contributor.author | Gessesse, Amare | |
dc.contributor.author | Colin Murrell, Murrell | |
dc.contributor.author | Øvreås, Lise | |
dc.date.accessioned | 2022-03-31T13:39:00Z | |
dc.date.available | 2022-03-31T13:39:00Z | |
dc.date.created | 2022-01-19T10:35:02Z | |
dc.date.issued | 2021-02-25 | |
dc.identifier.issn | 2076-2607 | |
dc.identifier.uri | https://hdl.handle.net/11250/2988923 | |
dc.description.abstract | Methylotrophic bacteria (non-methanotrophic methanol oxidizers) consuming reduced carbon compounds containing no carbon–carbon bonds as their sole carbon and energy source have been found in a great variety of environments. Here, we report a unique moderately thermophilic methanol-oxidising bacterium (strain LS7-MT) that grows optimally at 55 °C (with a growth range spanning 30 to 60 °C). The pure isolate was recovered from a methane-utilizing mixed culture enrichment from an alkaline thermal spring in the Ethiopia Rift Valley, and utilized methanol, methylamine, glucose and a variety of multi-carbon compounds. Phylogenetic analysis of the 16S rRNA gene sequences demonstrated that strain LS7-MT represented a new facultatively methylotrophic bacterium within the order Hyphomicrobiales of the class Alphaproteobacteria. This new strain showed 94 to 96% 16S rRNA gene identity to the two methylotroph genera, Methyloceanibacter and Methyloligella. Analysis of the draft genome of strain LS7-MT revealed genes for methanol dehydrogenase, essential for methanol oxidation. Functional and comparative genomics of this new isolate revealed genomic and physiological divergence from extant methylotrophs. Strain LS7-MT contained a complete mxaF gene cluster and xoxF1 encoding the lanthanide-dependent methanol dehydrogenase (XoxF). This is the first report of methanol oxidation at 55 °C by a moderately thermophilic bacterium within the class Alphaproteobacteria. These findings expand our knowledge of methylotrophy by the phylum Proteobacteria in thermal ecosystems and their contribution to global carbon and nitrogen cycles. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | MDPI | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | A novel moderately thermophilic facultative methylotroph within the class Alphaproteobacteria | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2021 the authors | en_US |
dc.source.articlenumber | 477 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.doi | 10.3390/microorganisms9030477 | |
dc.identifier.cristin | 1984402 | |
dc.source.journal | Microorganisms | en_US |
dc.identifier.citation | Microorganisms. 2021, 9 (3), 477. | en_US |
dc.source.volume | 9 | en_US |
dc.source.issue | 3 | en_US |