dc.contributor.author | Moyo, Sabrina John | |
dc.contributor.author | Manyahi, Joel | |
dc.contributor.author | Hubbard, Alasdair T. M | |
dc.contributor.author | Byrne, Rachel L | |
dc.contributor.author | Masoud, Nahya Salim | |
dc.contributor.author | Aboud, Said | |
dc.contributor.author | Manji, Karim | |
dc.contributor.author | Blomberg, Bjørn | |
dc.contributor.author | Langeland, Nina | |
dc.contributor.author | Roberts, Adam P. | |
dc.date.accessioned | 2022-04-20T10:56:16Z | |
dc.date.available | 2022-04-20T10:56:16Z | |
dc.date.created | 2021-10-14T15:44:25Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 0035-9203 | |
dc.identifier.uri | https://hdl.handle.net/11250/2991593 | |
dc.description.abstract | Background
We aimed to characterise the genetic determinants and context of two meropenem-resistant clinical isolates of Acinetobacter baumannii isolated from children hospitalised with bloodstream infections in Dar es Salaam, Tanzania.
Methods
Antimicrobial susceptibility was determined by disc diffusion E-test and broth microdilution. Genomes were completed using a hybrid assembly of Illumina and Oxford Nanopore Technologies sequencing reads and characterisation of the genetic context of resistance genes, multi-locus sequence types (STs) and phylogenetic analysis was determined bioinformatically.
Results
Twelve A. baumannii were isolated from 2226 blood cultures, two of which were meropenem-resistant. The two meropenem-resistant isolates, belonging to distinct STs, ST374 and ST239, were found to harbour blaNDM-1, which was chromosomally located in isolate DT0544 and plasmid-located in isolate DT01139. The genetic environment of blaNDM-1 shows the association of insertion sequence ISAba125 with blaNDM-1 in both isolates. Both isolates also harboured genes conferring resistance to other β-lactams, aminoglycosides and cotrimoxazole.
Conclusions
This is the first report of New Delhi metallo-β-lactamase-producing isolates of A. baumannii from Tanzania. The genetic context of blaNDM-1 provides further evidence of the importance of ISAba125 in the spread of blaNDM-1 in A. baumannii. Local surveillance should be strengthened to keep clinicians updated on the incidence of these and other multidrug-resistant and difficult-to-treat bacteria. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Oxford University Press | en_US |
dc.rights | Navngivelse-Ikkekommersiell 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/deed.no | * |
dc.title | Molecular characterisation of the first New Delhi metallo-β-lactamase 1-producing Acinetobacter baumannii from Tanzania | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2021 The Author(s) | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.doi | 10.1093/trstmh/traa173 | |
dc.identifier.cristin | 1946039 | |
dc.source.journal | Transactions of the Royal Society of Tropical Medicine and Hygiene | en_US |
dc.source.pagenumber | 1080-1085 | en_US |
dc.identifier.citation | Transactions of the Royal Society of Tropical Medicine and Hygiene. 2021, 115 (9), 1080-1085. | en_US |
dc.source.volume | 115 | en_US |
dc.source.issue | 9 | en_US |