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dc.contributor.authorJančuška, Alexander
dc.contributor.authorPotočárová, Alena
dc.contributor.authorKovalčíková, Alexandra Gaál
dc.contributor.authorPodracká, L’Udmila
dc.contributor.authorBabickova, Janka
dc.contributor.authorCelec, Peter
dc.contributor.authorTóthová, L’Ubomíra
dc.date.accessioned2022-08-09T10:28:39Z
dc.date.available2022-08-09T10:28:39Z
dc.date.created2022-04-13T14:47:22Z
dc.date.issued2022
dc.identifier.issn1661-6596
dc.identifier.urihttps://hdl.handle.net/11250/3010760
dc.description.abstractEarly and reliable markers of acute kidney injury (AKI) are essential. One such candidate marker of tissue damage is extracellular DNA (ecDNA). The aim of our present study is to describe the unknown dynamics of ecDNA in an animal model of AKI. Glycerol-induced nephropathy was used to model AKI in adult male Wistar rats (n = 93). Blood and urine samples were collected 1, 3, and 24 h after model induction. Total ecDNA and its sub-cellular origin was assessed. In the plasma, total ecDNA and nuclear ecDNA were significantly increased in the AKI group already after 1 h (160% and 270%, respectively, p = 0.02 and p = 0.04). Both nuclear and mitochondrial ecDNA were higher after 3 h (180% and 170%, respectively, p = 0.002 and p = 0.005). Urinary ecDNA concentrations in the AKI group were significantly increased only 24 h after model induction (130% for total ecDNA, p = 0.009; 210% for nuclear ecDNA, p = 0.02; and 200% for mitochondrial ecDNA, p = 0.0009). Our results indicate that plasma ecDNA has the potential to serve as an early and sensitive, albeit non-specific marker of AKI. Further studies should elucidate the source of ecDNA and the dynamics of ecDNA in other animal models of AKI and patients with AKI.en_US
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleDynamics of Plasma and Urinary Extracellular DNA in Acute Kidney Injuryen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2022 the authorsen_US
dc.source.articlenumber3402en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.doi10.3390/ijms23063402
dc.identifier.cristin2017222
dc.source.journalInternational Journal of Molecular Sciencesen_US
dc.identifier.citationInternational Journal of Molecular Sciences. 2022, 23 (6), 3402.en_US
dc.source.volume23en_US
dc.source.issue6en_US


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal