dc.contributor.author | Sushentsev, Nikita | |
dc.contributor.author | McLean, Mary A. | |
dc.contributor.author | Warren, Anne Y. | |
dc.contributor.author | Benjamin, Arnold J. V. | |
dc.contributor.author | Brodie, Cara | |
dc.contributor.author | Frary, Amy | |
dc.contributor.author | Gill, Andrew B. | |
dc.contributor.author | Jones, Julia | |
dc.contributor.author | Kaggie, Joshua D. | |
dc.contributor.author | Lamb, Benjamin W. | |
dc.contributor.author | Locke, Matthew J. | |
dc.contributor.author | Miller, Jodi L. | |
dc.contributor.author | Mills, Ian | |
dc.contributor.author | Priest, Andrew N. | |
dc.contributor.author | Robb, Fraser J. L. | |
dc.contributor.author | Shah, Nimish | |
dc.contributor.author | Schulte, Rolf S. | |
dc.contributor.author | Graves, Martin J. | |
dc.contributor.author | Gnanapragasam, Vincent J. | |
dc.contributor.author | Brindle, Kevin M. | |
dc.contributor.author | Barrett, Tristan | |
dc.contributor.author | Gallagher, Ferdia A. | |
dc.date.accessioned | 2022-08-30T12:30:21Z | |
dc.date.available | 2022-08-30T12:30:21Z | |
dc.date.created | 2022-05-12T13:04:54Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://hdl.handle.net/11250/3014395 | |
dc.description.abstract | Hyperpolarised magnetic resonance imaging (HP 13C-MRI) is an emerging clinical technique to detect [1-13C]lactate production in prostate cancer (PCa) following intravenous injection of hyperpolarised [1-13C]pyruvate. Here we differentiate clinically significant PCa from indolent disease in a low/intermediate-risk population by correlating [1-13C]lactate labelling on MRI with the percentage of Gleason pattern 4 (%GP4) disease. Using immunohistochemistry and spatial transcriptomics, we show that HP 13C-MRI predominantly measures metabolism in the epithelial compartment of the tumour, rather than the stroma. MRI-derived tumour [1-13C]lactate labelling correlated with epithelial mRNA expression of the enzyme lactate dehydrogenase (LDHA and LDHB combined), and the ratio of lactate transporter expression between the epithelial and stromal compartments (epithelium-to-stroma MCT4). We observe similar changes in MCT4, LDHA, and LDHB between tumours with primary Gleason patterns 3 and 4 in an independent TCGA cohort. Therefore, HP 13C-MRI can metabolically phenotype clinically significant disease based on underlying metabolic differences in the epithelial and stromal tumour compartments. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Nature Research | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Hyperpolarised 13C-MRI identifies the emergence of a glycolytic cell population within intermediaterisk human prostate cancer | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright The Author(s) 2022, corrected publication 2022 | en_US |
dc.source.articlenumber | 466 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.doi | 10.1038/s41467-022-28069-2 | |
dc.identifier.cristin | 2023914 | |
dc.source.journal | Nature Communications | en_US |
dc.identifier.citation | Nature Communications. 2022, 13, 466. | en_US |
dc.source.volume | 13 | en_US |