dc.contributor.author | Namløs, Heidi Maria | |
dc.contributor.author | Skårn, Magne Nordang | |
dc.contributor.author | Ali, Deeqa Ahmed Mohamed | |
dc.contributor.author | Grad, Iwona | |
dc.contributor.author | Andresen, Kim | |
dc.contributor.author | Kresse, Stine Henrichson | |
dc.contributor.author | Munthe, Else | |
dc.contributor.author | Serra, Massimo | |
dc.contributor.author | Scotlandi, Katia | |
dc.contributor.author | Llombart-Bosch, Antonio | |
dc.contributor.author | Myklebost, Ola | |
dc.contributor.author | Lind, Guro Elisabeth | |
dc.contributor.author | Meza, Leonardo Zepeda | |
dc.date.accessioned | 2022-10-10T13:35:01Z | |
dc.date.available | 2022-10-10T13:35:01Z | |
dc.date.created | 2022-05-23T10:09:27Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 1471-2164 | |
dc.identifier.uri | https://hdl.handle.net/11250/3025159 | |
dc.description.abstract | Background
Osteosarcoma is the most common primary malignant tumour of bone occurring in children and young adolescents and is characterised by complex genetic and epigenetic changes. The miRNA miR-486-5p has been shown to be downregulated in osteosarcoma and in cancer in general.
Results
To investigate if the mir-486 locus is epigenetically regulated, we integrated DNA methylation and miR-486-5p expression data using cohorts of osteosarcoma cell lines and patient samples. A CpG island in the promoter of the ANK1 host gene of mir-486 was shown to be highly methylated in osteosarcoma cell lines as determined by methylation-specific PCR and direct bisulfite sequencing. High methylation levels were seen for osteosarcoma patient samples, xenografts and cell lines based on quantitative methylation-specific PCR. 5-Aza-2′-deoxycytidine treatment of osteosarcoma cell lines caused induction of miR-486-5p and ANK1, indicating common epigenetic regulation in osteosarcoma cell lines. When overexpressed, miR-486-5p affected cell morphology.
Conclusions
miR-486-5p represents a highly cancer relevant, epigenetically regulated miRNA in osteosarcoma, and this knowledge contributes to the understanding of osteosarcoma biology. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | BMC | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | miR-486-5p expression is regulated by DNA methylation in osteosarcoma | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2022 The Author(s) | en_US |
dc.source.articlenumber | 142 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.doi | 10.1186/s12864-022-08346-6 | |
dc.identifier.cristin | 2026392 | |
dc.source.journal | BMC Genomics | en_US |
dc.identifier.citation | BMC Genomics. 2022, 23, 142. | en_US |
dc.source.volume | 23 | en_US |