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dc.contributor.authorWilson, Madison N.
dc.contributor.authorThunemann, Martin
dc.contributor.authorLiu, Xin
dc.contributor.authorLu, Yichen
dc.contributor.authorPuppo, Francesca
dc.contributor.authorAdams, Jason W.
dc.contributor.authorKim, Jeong-Hoon
dc.contributor.authorRamezani, Mehrdad
dc.contributor.authorPizzo, Donald P.
dc.contributor.authorDjurovic, Srdjan
dc.contributor.authorAndreassen, Ole
dc.contributor.authorMansour, Abed AlFatah
dc.contributor.authorGage, Fred H.
dc.contributor.authorMuotri, Alysson R.
dc.contributor.authorDevor, Anna
dc.contributor.authorKuzum, Duygu
dc.date.accessioned2023-01-30T13:48:49Z
dc.date.available2023-01-30T13:48:49Z
dc.date.created2023-01-10T13:03:13Z
dc.date.issued2022
dc.identifier.issn2041-1723
dc.identifier.urihttps://hdl.handle.net/11250/3047188
dc.description.abstractHuman cortical organoids, three-dimensional neuronal cultures, are emerging as powerful tools to study brain development and dysfunction. However, whether organoids can functionally connect to a sensory network in vivo has yet to be demonstrated. Here, we combine transparent microelectrode arrays and two-photon imaging for longitudinal, multimodal monitoring of human cortical organoids transplanted into the retrosplenial cortex of adult mice. Two-photon imaging shows vascularization of the transplanted organoid. Visual stimuli evoke electrophysiological responses in the organoid, matching the responses from the surrounding cortex. Increases in multi-unit activity (MUA) and gamma power and phase locking of stimulus-evoked MUA with slow oscillations indicate functional integration between the organoid and the host brain. Immunostaining confirms the presence of human-mouse synapses. Implantation of transparent microelectrodes with organoids serves as a versatile in vivo platform for comprehensive evaluation of the development, maturation, and functional integration of human neuronal networks within the mouse brain.en_US
dc.language.isoengen_US
dc.publisherNatureen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleMultimodal monitoring of human cortical organoids implanted in mice reveal functional connection with visual cortexen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.source.articlenumber7945en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2
dc.identifier.doi10.1038/s41467-022-35536-3
dc.identifier.cristin2104103
dc.source.journalNature Communicationsen_US
dc.identifier.citationNature Communications. 2022, 13, 7945.en_US
dc.source.volume13en_US


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal