dc.contributor.author | Wilson, Madison N. | |
dc.contributor.author | Thunemann, Martin | |
dc.contributor.author | Liu, Xin | |
dc.contributor.author | Lu, Yichen | |
dc.contributor.author | Puppo, Francesca | |
dc.contributor.author | Adams, Jason W. | |
dc.contributor.author | Kim, Jeong-Hoon | |
dc.contributor.author | Ramezani, Mehrdad | |
dc.contributor.author | Pizzo, Donald P. | |
dc.contributor.author | Djurovic, Srdjan | |
dc.contributor.author | Andreassen, Ole | |
dc.contributor.author | Mansour, Abed AlFatah | |
dc.contributor.author | Gage, Fred H. | |
dc.contributor.author | Muotri, Alysson R. | |
dc.contributor.author | Devor, Anna | |
dc.contributor.author | Kuzum, Duygu | |
dc.date.accessioned | 2023-01-30T13:48:49Z | |
dc.date.available | 2023-01-30T13:48:49Z | |
dc.date.created | 2023-01-10T13:03:13Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://hdl.handle.net/11250/3047188 | |
dc.description.abstract | Human cortical organoids, three-dimensional neuronal cultures, are emerging as powerful tools to study brain development and dysfunction. However, whether organoids can functionally connect to a sensory network in vivo has yet to be demonstrated. Here, we combine transparent microelectrode arrays and two-photon imaging for longitudinal, multimodal monitoring of human cortical organoids transplanted into the retrosplenial cortex of adult mice. Two-photon imaging shows vascularization of the transplanted organoid. Visual stimuli evoke electrophysiological responses in the organoid, matching the responses from the surrounding cortex. Increases in multi-unit activity (MUA) and gamma power and phase locking of stimulus-evoked MUA with slow oscillations indicate functional integration between the organoid and the host brain. Immunostaining confirms the presence of human-mouse synapses. Implantation of transparent microelectrodes with organoids serves as a versatile in vivo platform for comprehensive evaluation of the development, maturation, and functional integration of human neuronal networks within the mouse brain. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Nature | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Multimodal monitoring of human cortical organoids implanted in mice reveal functional connection with visual cortex | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2022 The Author(s) | en_US |
dc.source.articlenumber | 7945 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.doi | 10.1038/s41467-022-35536-3 | |
dc.identifier.cristin | 2104103 | |
dc.source.journal | Nature Communications | en_US |
dc.identifier.citation | Nature Communications. 2022, 13, 7945. | en_US |
dc.source.volume | 13 | en_US |