Elucidating tumour-associated microglia/macrophage diversity along glioblastoma progression and under ACOD1 deficiency
Pires-Afonso, Yolanda; Muller, Arnaud; Grzyb, Kamil; Oudin, Anaïs; Yabo, Yahaya A.; Sousa, Carole; Scafidi, Andrea; Poli, Aurélie; Cosma, Antonio; Halder, Rashi; Coowar, Djalil; Golebiewska, Anna; Skupin, Alexander; Niclou, Simone Pierrette; Michelucci, Alessandro
Journal article, Peer reviewed
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https://hdl.handle.net/11250/3058430Utgivelsesdato
2022Metadata
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Sammendrag
In glioblastoma (GBM), tumour-associated microglia/macrophages (TAMs) represent the major cell type of the stromal compartment and contribute to tumour immune escape mechanisms. Thus, targeting TAMs is emerging as a promising strategy for immunotherapy. However, TAM heterogeneity and metabolic adaptation along GBM progression represent critical features for the design of effective TAM-targeted therapies. Here, we comprehensively study the cellular and molecular changes of TAMs in the GL261 GBM mouse model, combining single-cell RNA-sequencing with flow cytometry and immunohistological analyses along GBM progression and in the absence of Acod1 (also known as Irg1), a key gene involved in the metabolic reprogramming of macrophages towards an anti-inflammatory phenotype. Similarly to patients, we identify distinct TAM profiles, mainly based on their ontogeny, that reiterate the idea that microglia- and macrophage-like cells show key transcriptional differences and dynamically adapt along GBM stages. Notably, we uncover decreased antigen-presenting cell features and immune reactivity in TAMs along tumour progression that are instead enhanced in Acod1-deficient mice. Overall, our results provide insight into TAM heterogeneity and highlight a novel role for Acod1 in TAM adaptation during GBM progression.