dc.contributor.author | Barakat, Ahmed Samy Mohamed Ali | |
dc.contributor.author | Birkeland, Even | |
dc.contributor.author | Jørstad, Melissa Davidsen | |
dc.contributor.author | el Hajj, Magalie | |
dc.contributor.author | Marijani, Msafiri | |
dc.contributor.author | Døskeland, Anne Marie Simonne | |
dc.contributor.author | Mjaavatten, Olav | |
dc.contributor.author | Berven, Frode Steingrimsen | |
dc.contributor.author | Mustafa, Tehmina | |
dc.date.accessioned | 2024-01-16T12:39:53Z | |
dc.date.available | 2024-01-16T12:39:53Z | |
dc.date.created | 2023-06-07T08:02:45Z | |
dc.date.issued | 2023 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | https://hdl.handle.net/11250/3111841 | |
dc.description.abstract | This study aimed at exploring the proteomic profile of PBMCs to predict treatment response in pulmonary tuberculosis (PTB). This was a pilot study conducted among 8 adult patients from Zanzibar, Tanzania with confirmed PTB. Blood samples were collected at baseline, at 2 months of treatment, and at the end of treatment at 6 months. Proteins were extracted from PBMCs and analyzed using LC-MS/MS based label free quantitative proteomics. Overall, 3,530 proteins were quantified across the samples, and 12 differentially expressed proteins were identified at both 2 months of treatment and at treatment completion, which were involved in cellular and metabolic processes, as well as binding and catalytic activity. Seven were downregulated proteins (HSPA1B/HSPA1A, HSPH1, HSP90AA1, lipopolysaccharide-binding protein, complement component 9, calcyclin-binding protein, and protein transport protein Sec31A), and 5 proteins were upregulated (SEC14 domain and spectrin repeat-containing protein 1, leucine-rich repeat-containing 8 VRAC subunit D, homogentisate 1,2-dioxygenase, NEDD8-activating enzyme E1 regulatory subunit, and N-acetylserotonin O-methyltransferase-like protein). The results showed that proteome analysis of PBMCs can be used as a novel technique to identify protein abundance change with anti-tuberculosis treatment. The novel proteins elucidated in this work may provide new insights for understanding PTB pathogenesis, treatment, and prognosis. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | PLoS | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Proteomic analysis of peripheral blood mononuclear cells isolated from patients with pulmonary tuberculosis: A pilot study from Zanzibar, Tanzania | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2023 The Author(s) | en_US |
dc.source.articlenumber | e0281757 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.doi | 10.1371/journal.pone.0281757 | |
dc.identifier.cristin | 2152402 | |
dc.source.journal | PLOS ONE | en_US |
dc.identifier.citation | PLOS ONE. 2023, 18 (2), e0281757. | en_US |
dc.source.volume | 18 | en_US |
dc.source.issue | 2 | en_US |