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dc.contributor.authorShumba, Patience
dc.contributor.authorSura, Thomas
dc.contributor.authorMoll, Kirsten
dc.contributor.authorChakrakodi, Bhavya
dc.contributor.authorTölken, Lea A.
dc.contributor.authorHoßmann, Jörn
dc.contributor.authorHoff, Katharina J.
dc.contributor.authorHyldegaard, Ole
dc.contributor.authorNekludov, Michael
dc.contributor.authorSvensson, Mattias
dc.contributor.authorArnell, Per
dc.contributor.authorSkrede, Steinar
dc.contributor.authorNorrby-Teglund, Anna
dc.contributor.authorSiemens, Nikolai
dc.description.abstractBackground Streptococcus pyogenes (group A streptococci; GAS) is the main causative pathogen of monomicrobial necrotizing soft tissue infections (NSTIs). To resist immuno-clearance, GAS adapt their genetic information and/or phenotype to the surrounding environment. Hyper-virulent streptococcal pyrogenic exotoxin B (SpeB) negative variants caused by covRS mutations are enriched during infection. A key driving force for this process is the bacterial Sda1 DNase. Methods Bacterial infiltration, immune cell influx, tissue necrosis and inflammation in patient´s biopsies were determined using immunohistochemistry. SpeB secretion and activity by GAS post infections or challenges with reactive agents were determined via Western blot or casein agar and proteolytic activity assays, respectively. Proteome of GAS single colonies and neutrophil secretome were profiled, using mass spectrometry. Results Here, we identify another strategy resulting in SpeB-negative variants, namely reversible abrogation of SpeB secretion triggered by neutrophil effector molecules. Analysis of NSTI patient tissue biopsies revealed that tissue inflammation, neutrophil influx, and degranulation positively correlate with increasing frequency of SpeB-negative GAS clones. Using single colony proteomics, we show that GAS isolated directly from tissue express but do not secrete SpeB. Once the tissue pressure is lifted, GAS regain SpeB secreting function. Neutrophils were identified as the main immune cells responsible for the observed phenotype. Subsequent analyses identified hydrogen peroxide and hypochlorous acid as reactive agents driving this phenotypic GAS adaptation to the tissue environment. SpeB-negative GAS show improved survival within neutrophils and induce increased degranulation. Conclusions Our findings provide new information about GAS fitness and heterogeneity in the soft tissue milieu and provide new potential targets for therapeutic intervention in NSTIs.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleNeutrophil-derived reactive agents induce a transient SpeB negative phenotype in Streptococcus pyogenesen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.rights.holderCopyright 2023 The Author(s)en_US
dc.source.journalJournal of Biomedical Scienceen_US
dc.identifier.citationJournal of Biomedical Science. 2023, 30 (1), 52.en_US

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Navngivelse 4.0 Internasjonal
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