dc.contributor.author | Markussen, Dagfinn Lunde | |
dc.contributor.author | Ebbesen, Marit Helen | |
dc.contributor.author | Serigstad, Sondre | |
dc.contributor.author | Knoop, Siri Tandberg | |
dc.contributor.author | Ritz, Christian | |
dc.contributor.author | Bjørneklett, Rune Oskar | |
dc.contributor.author | Kommedal, Øyvind | |
dc.contributor.author | Jenum, Synne | |
dc.contributor.author | Ulvestad, Elling | |
dc.contributor.author | Grewal, Harleen | |
dc.date.accessioned | 2024-05-07T13:59:03Z | |
dc.date.available | 2024-05-07T13:59:03Z | |
dc.date.created | 2023-11-23T13:37:28Z | |
dc.date.issued | 2023 | |
dc.identifier.issn | 2165-0497 | |
dc.identifier.uri | https://hdl.handle.net/11250/3129564 | |
dc.description.abstract | This prospective study assessed the value of initial microscopy evaluation of sputum samples submitted for rapid syndromic PCR-based testing. Bacterial detections by the BioFire FilmArray Pneumonia Panel plus in 126 high- and 108 low-quality sputum samples, based on initial microscopy evaluation in samples from patients with lower respiratory tract infections were compared. We found that high-quality samples had a higher proportion of bacterial detections compared to low-quality samples (P = 0.013). This included a higher proportion of detections of bacteria deemed clinically relevant by predefined criteria (70% and 55%, P = 0.016), as well as a higher proportion of detections of Haemophilus influenzae (36% and 20%, P = 0.010). High-quality samples also had more detections of bacteria with high semi-quantitative values. The study found no significant difference between high- and low-quality samples in the proportions of samples with a single species of bacteria detected, samples with a bacteria treated by the clinician, samples with detection of a proven etiology of community-acquired pneumonia by predefined criteria, the number of bacterial species detected, or the detection of Streptococcus pneumoniae, Moraxella catarrhalis, or Staphylococcus aureus. The results showed that 40% (95% CI 35%–47%) of the bacterial detections would have been missed if only high-quality samples were analyzed. This included 41% (27%–56%) of detections of S. pneumoniae, 33% (23%–45%) of detections of H. influenzae, 42% (28%–58%) of detections of S. aureus, and 37% (23%–54%) of detections of M. catarrhalis. These findings suggest that all sputum samples submitted for rapid syndromic PCR testing should be analyzed, regardless of initial microscopy quality assessment. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | American Society for Microbiology | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | The diagnostic utility of microscopic quality assessment of sputum samples in the era of rapid syndromic PCR testing | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2023 the authors | en_US |
dc.source.articlenumber | e03002-23 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.doi | 10.1128/spectrum.03002-23 | |
dc.identifier.cristin | 2201062 | |
dc.source.journal | Microbiology spectrum | en_US |
dc.identifier.citation | Microbiology spectrum. 2023, 11 (5), e03002-23. | en_US |
dc.source.volume | 11 | en_US |
dc.source.issue | 5 | en_US |