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dc.contributor.authorSønstevold, Tonjeen_US
dc.contributor.authorJohannessen, Anne Christineen_US
dc.contributor.authorStuhr, Linda Elin Birkhaugen_US
dc.PublishedRadiation Oncology 2015, 10:129eng
dc.description.abstractBackground Radiation technology focuses on delivering the radiation as precisely as possible to the tumor, nonetheless both acute and long-term damage to surrounding normal tissue may develop. Injuries to the surrounding normal tissue after radiotherapy of head and neck cancer are difficult to manage. An animal model is needed to elucidate good treatment modalities. The aim of this study was to establish a rat model where a certain radiation dose gives reproducible tissue reactions in the mandibular area corresponding to injuries obtained in humans. Method The left mandible of male Sprague Dawley rats was irradiated by external radiotherapy (single fraction 15 Gy, total dose 75 Gy) every second week five times. Endpoint was six weeks after last radiation treatment, and the test group was compared to non-irradiated controls. Morphological alterations of the soft tissues, bone and tooth formation, as well as alterations of salivation, vascularity and collagen content were assessed. An unpaired, non-parametric Mann–Whitney test was used to compare the statistical differences between the groups. Results Analysis of the soft tissues and mandible within the radiation field revealed severe unilateral alopecia and dermatitis of the skin, extensive inflammation of the submandibular gland with loss of serous secretory cells, hyperkeratinization and dense connective fiber bundles of the gingival tissue, and disturbed tooth development with necrosis of the pulp. Production of saliva and the vascularity of the soft tissues were significantly reduced. Furthermore, the collagen fibril diameter was larger and the collagen network denser compared to non-irradiated control rats. Conclusion We have established an animal model of radiation injury demonstrating physiological and histological changes corresponding to human radiation injuries, which can be used for future therapeutic evaluations.en_US
dc.publisherBioMed Centraleng
dc.rightsAttribution CC BYeng
dc.subjectRadiation injuryeng
dc.subjectHead and neck cancereng
dc.titleA rat model of radiation injury in the mandibular areaen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright Sønstevold et al. 2015
dc.subject.nsiVDP::Medisinske fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Generell patologi, patologisk anatomi : 719
dc.subject.nsiVDP::Midical sciences: 700::Basic medical, dental and veterinary sciences: 710::General pathology, anatomical pathology: 719
dc.subject.nsiVDP::Medisinske fag: 700::Klinisk medisinske fag: 750::Onkologi: 762
dc.subject.nsiVDP::Midical sciences: 700::Clinical medical sciences: 750::Oncology: 762
dc.subject.nsiVDP::Medisinske Fag: 700en_US

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