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dc.contributor.authorGrindheim, Ann Karien_US
dc.contributor.authorHollås, Hanneen_US
dc.contributor.authorRaddum, Aase Meretheen_US
dc.contributor.authorSaraste, Jaakkoen_US
dc.contributor.authorVedeler, Annien_US
dc.PublishedJournal of Cell Science 2015eng
dc.description.abstractAnnexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly regulated by its post-translational modifications. AnxA2 and its Tyr23-phosphorylated form (pTyr23AnxA2) are involved in malignant cell transformation, metastasis and angiogenesis. Here, we show that H2O2 exerts rapid, simultaneous and opposite effects on the Tyr23 phosphorylation status of AnxA2 in two distinct compartments of rat pheochromocytoma (PC12) cells. Reactive oxygen species induce dephosphorylation of pTyr23AnxA2 located in the PML bodies of the nucleus, whereas AnxA2 associated with F-actin at the cell cortex is Tyr23 phosphorylated. The H2O2-induced responses in both compartments are transient and the pTyr23AnxA2 accumulating at the cell cortex is subsequently incorporated into vesicles and then released to the extracellular space. Blocking nuclear export by leptomycin B does not affect the nuclear pool of pTyr23AnxA2, but increases the amount of total AnxA2 in this compartment, indicating that the protein might have several functions in the nucleus. These results suggest that Tyr23 phosphorylation can regulate the function of AnxA2 at distinct subcellular sites.en_US
dc.publisherThe Company of Biologists Ltd.eng
dc.rightsAttribution CC BY 3.0eng
dc.subjectAnnexin A2eng
dc.subjectTyrosine phosphorylationeng
dc.subjectOxidative stresseng
dc.subjectCell cortexeng
dc.titleReactive oxygen species exert opposite effects on Tyr23 phosphorylation of the nuclear and cortical pools of Annexin A2en_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2016
dc.subject.nsiVDP::Medisinske Fag: 700en_US

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Attribution CC BY 3.0
Med mindre annet er angitt, så er denne innførselen lisensiert som Attribution CC BY 3.0