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dc.contributor.authorAye, Thin Thinen_US
dc.contributor.authorLow, Teck Yewen_US
dc.contributor.authorBjørlykke, Yngvilden_US
dc.contributor.authorBarsnes, Haralden_US
dc.contributor.authorHeck, Albert J.R.en_US
dc.contributor.authorBerven, Frodeen_US
dc.date.accessioned2017-09-19T13:30:40Z
dc.date.available2017-09-19T13:30:40Z
dc.date.issued2012
dc.PublishedAye TT, Low, Bjørlykke Y, Barsnes H, Heck AJ, Berven F. Use of stable isotope dimethyl labeling coupled to selected reaction monitoring to enhance throughput by multiplexing relative quantitation of targeted proteins. Analytical Chemistry. 2012;84(11):4999-5006eng
dc.identifier.issn1520-6882
dc.identifier.issn0003-2700
dc.identifier.urihttps://hdl.handle.net/1956/16673
dc.description.abstractIn this manuscript, we present a proof-of-concept study for targeted relative protein quantitation workflow using chemical labeling in the form of dimethylation, coupled with selected reaction monitoring (dimethyl-SRM). We first demonstrate close to complete isotope incorporation for all peptides tested. The accuracy, reproducibility, and linear dynamic range of quantitation are further assessed based on known ratios of nonhuman standard proteins spiked into human cerebrospinal fluid (CSF) as a model complex matrix. Quantitation reproducibility below 20% (CV < 20%) was obtained for analyte concentrations present at a dynamic range of 4 orders of magnitude lower than that of the background proteins. An error of less than 15% was observed when measuring the abundance of 44 out of 45 major human plasma proteins. Dimethyl-SRM was further examined by comparing the relative quantitation of eight proteins in human CSF with the relative quantitation obtained using synthetic heavy peptides coupled to stable isotope dilution-SRM (SID-SRM). Comparison between the two methods reveals that the correlation between dimethyl-SRM and SID-SRM is within 0.3–33% variation, demonstrating the accuracy of relative quantitation using dimethyl-SRM. Dimethyl labeling coupled with SRM provides a fast, convenient, and cost-effective alternative for relative quantitation of a large number of candidate proteins/peptides.en_US
dc.language.isoengeng
dc.publisherAmerican Chemical Societyeng
dc.titleUse of stable isotope dimethyl labeling coupled to selected reaction monitoring to enhance throughput by multiplexing relative quantitation of targeted proteinsen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2017-09-06T14:32:26Z
dc.description.versionacceptedVersionen_US
dc.rights.holderCopyright 2012 American Chemical Society
dc.identifier.doihttps://doi.org/10.1021/ac300596r
dc.identifier.cristin947705
dc.source.journalAnalytical Chemistry
dc.relation.projectNorges forskningsråd: 204833
dc.subject.nsiVDP::Medisinske fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk biokjemi: 726
dc.subject.nsiVDP::Midical sciences: 700::Basic medical, dental and veterinary sciences: 710::Medical biochemistry: 726


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