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dc.contributor.authorPavlin, Tinaen_US
dc.contributor.authorNagelhus, Erlend Arnulfen_US
dc.contributor.authorBrekken, Christianen_US
dc.contributor.authorEyjolfsson, Elvar M.en_US
dc.contributor.authorThoren, Annaen_US
dc.contributor.authorHaraldseth, Olaven_US
dc.contributor.authorSonnewald, Ursulaen_US
dc.contributor.authorOttersen, Ole Petteren_US
dc.contributor.authorHåberg, Astaen_US
dc.date.accessioned2017-12-18T14:31:07Z
dc.date.available2017-12-18T14:31:07Z
dc.date.issued2017-01
dc.PublishedPavlin T, Nagelhus EA, Brekken C, Eyjolfsson E.M, Thoren A, Haraldseth O, Sonnewald U, Ottersen OP, Håberg A. Loss or mislocalization of aquaporin-4 affects diffusion properties and intermediary metabolism in gray matter of mice. Neurochemical Research. 2017;42(1):77-91eng
dc.identifier.issn0364-3190
dc.identifier.issn1573-6903
dc.identifier.urihttps://hdl.handle.net/1956/17021
dc.description.abstractThe first aim of this study was to determine how complete or perivascular loss of aquaporin-4 (AQP4) water channels affects membrane permeability for water in the mouse brain grey matter in the steady state. Time-dependent diffusion magnetic resonance imaging was performed on global Aqp4 knock out (KO) and α-syntrophin (α-syn) KO mice, in the latter perivascular AQP4 are mislocalized, but still functioning. Control animals were corresponding wild type (WT) mice. By combining in vivo diffusion measurements with the effective medium theory and previously measured extra-cellular volume fractions, the effects of membrane permeability and extracellular volume fraction were uncoupled for Aqp4 and α-syn KO. The second aim was to assess the effect of α-syn KO on cortical intermediary metabolism combining in vivo [1-13C]glucose and [1,2-13C]acetate injection with ex vivo 13C MR spectroscopy. Aqp4 KO increased the effective diffusion coefficient at long diffusion times by 5%, and a 14% decrease in membrane water permeability was estimated for Aqp4 KO compared with WT mice. α-syn KO did not affect the measured diffusion parameters. In the metabolic analyses, significantly lower amounts of [4-13C]glutamate and [4-13C]glutamine, and percent enrichment in [4-13C]glutamate were detected in the α-syn KO mice. [1,2-13C]acetate metabolism was unaffected in α-syn KO, but the contribution of astrocyte derived metabolites to GABA synthesis was significantly increased. Taken together, α-syn KO mice appeared to have decreased neuronal glucose metabolism, partly compensated for by utilization of astrocyte derived metabolites.en_US
dc.language.isoengeng
dc.publisherSpringereng
dc.subjectCortexeng
dc.subjectDiffusion weighted MRIeng
dc.subject13C MRSeng
dc.subjectGlutamateeng
dc.subjectGlucoseeng
dc.subjectMembrane permeabilityeng
dc.titleLoss or mislocalization of aquaporin-4 affects diffusion properties and intermediary metabolism in gray matter of miceen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2017-11-12T11:41:52Z
dc.description.versionacceptedVersionen_US
dc.rights.holderCopyright 2016 Springer Science+Business Media New York
dc.identifier.doihttps://doi.org/10.1007/s11064-016-2139-y
dc.identifier.cristin1439345
dc.source.journalNeurochemical Research


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