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dc.contributor.authorMuruganandam, Gopinathen_US
dc.contributor.authorRaasakka, Arneen_US
dc.contributor.authorMyllykoski, Mattien_US
dc.contributor.authorKursula, Inarien_US
dc.contributor.authorKursula, Petrien_US
dc.PublishedMuruganandam G, Raasakka A, Myllykoski M, Kursula I, Kursula P. Structural similarities and functional differences clarify evolutionary relationships between tRNA healing enzymes and the myelin enzyme CNPase. BMC Biochemistry. 2017;18:7eng
dc.description.abstractBackground: Eukaryotic tRNA splicing is an essential process in the transformation of a primary tRNA transcript into a mature functional tRNA molecule. 5′-phosphate ligation involves two steps: a healing reaction catalyzed by polynucleotide kinase (PNK) in association with cyclic phosphodiesterase (CPDase), and a sealing reaction catalyzed by an RNA ligase. The enzymes that catalyze tRNA healing in yeast and higher eukaryotes are homologous to the members of the 2H phosphoesterase superfamily, in particular to the vertebrate myelin enzyme 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase). Results: We employed different biophysical and biochemical methods to elucidate the overall structural and functional features of the tRNA healing enzymes yeast Trl1 PNK/CPDase and lancelet PNK/CPDase and compared them with vertebrate CNPase. The yeast and the lancelet enzymes have cyclic phosphodiesterase and polynucleotide kinase activity, while vertebrate CNPase lacks PNK activity. In addition, we also show that the healing enzymes are structurally similar to the vertebrate CNPase by applying synchrotron radiation circular dichroism spectroscopy and small-angle X-ray scattering. Conclusions: We provide a structural analysis of the tRNA healing enzyme PNK and CPDase domains together. Our results support evolution of vertebrate CNPase from tRNA healing enzymes with a loss of function at its N-terminal PNK-like domain.en_US
dc.publisherBioMed Centraleng
dc.rightsAttribution CC BYeng
dc.subjectCyclic phosphodiesteraseeng
dc.subjectPolynucleotide kinaseeng
dc.subjectSubstrate specificityeng
dc.subjectProtein structureeng
dc.subject2H familyeng
dc.subjecttRNA splicingeng
dc.titleStructural similarities and functional differences clarify evolutionary relationships between tRNA healing enzymes and the myelin enzyme CNPaseen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2017 The Author(s)
dc.source.journalBMC Biochemistry

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