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dc.contributor.authorHartveit, Espenen_US
dc.contributor.authorVeruki, Margaret Linen_US
dc.contributor.authorZandt, Bas-Janen_US
dc.PublishedHartveit E, Veruki ML, Zandt B. Capacitance measurement of dendritic exocytosis in an electrically coupled inhibitory retinal interneuron: an experimental and computational study. Physiological Reports. 2019;7(15).eng
dc.description.abstractExocytotic release of neurotransmitter can be quantified by electrophysiological recording from postsynaptic neurons. Alternatively, fusion of synaptic vesicles with the cell membrane can be measured as increased capacitance by recording directly from a presynaptic neuron. The “Sine + DC” technique is based on recording from an unbranched cell, represented by an electrically equivalent RC-circuit. It is challenging to extend such measurements to branching neurons where exocytosis occurs at a distance from a somatic recording electrode. The AII amacrine is an important inhibitory interneuron of the mammalian retina and there is evidence that exocytosis at presynaptic lobular dendrites increases the capacitance. Here, we combined electrophysiological recording and computer simulations with realistic compartmental models to explore capacitance measurements of rat AII amacrine cells. First, we verified the ability of the “Sine + DC” technique to detect depolarizationevoked exocytosis in physiological recordings. Next, we used compartmental modeling to demonstrate that capacitance measurements can detect increased membrane surface area at lobular dendrites. However, the accuracy declines for lobular dendrites located further from the soma due to frequency-dependent signal attenuation. For sine wave frequencies ≥1 kHz, the magnitude of the total releasable pool of synaptic vesicles will be significantly underestimated. Reducing the sine wave frequency increases overall accuracy, but when the frequency is sufficiently low that exocytosis can be detected with high accuracy from all lobular dendrites (~100 Hz), strong electrical coupling between AII amacrines compromises the measurements. These results need to be taken into account in studies with capacitance measurements from these and other electrically coupled neurons.en_US
dc.publisherWiley Open Accesseng
dc.rightsAttribution CC BYeng
dc.subjectAII amacrine celleng
dc.subjectcompartmental modeleng
dc.subjectinhibitory interneuroneng
dc.titleCapacitance measurement of dendritic exocytosis in an electrically coupled inhibitory retinal interneuron: an experimental and computational studyen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2019 The Authors.
dc.source.journalPhysiological Reports
dc.relation.projectNorges forskningsråd: 261914
dc.relation.projectNorges forskningsråd: 214216
dc.relation.projectNorges forskningsråd: 213776
dc.relation.projectNorges forskningsråd: 182743

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