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dc.contributor.authorVaudel, Marcen_US
dc.contributor.authorSickmann, Alberten_US
dc.contributor.authorMartens, Lennarten_US
dc.PublishedVaudel M, Sickmann A, Martens L. Introduction to opportunities and pitfalls in functional mass spectrometry based proteomics. Biochimica et Biophysica Acta - Proteins and Proteomics. 2014;1844(1):12-20eng
dc.description.abstractWith the advent of mass spectrometry based proteomics, the identification of thousands of proteins has become commonplace in biology nowadays. Increasingly, efforts have also been invested toward the detection and localization of posttranslational modifications. It is furthermore common practice to quantify the identified entities, a task supported by a panel of different methods. Finally, the results can also be enriched with functional knowledge gained on the proteins, detecting for instance differentially expressed gene ontology terms or biological pathways. In this study, we review the resources, methods and tools available for the researcher to achieve such a quantitative functional analysis. These include statistics for the post-processing of identification and quantification results, online resources and public repositories. With a focus on free but user-friendly software, preferably also open-source, we provide a list of tools designed to help the researcher manage the vast amount of data generated. We also indicate where such applications currently remain lacking. Moreover, we stress the eventual pitfalls of every step of such studies. This article is part of a Special Issue entitled: Computational Proteomics in the Post-Identification Era. Guest Editors: Martin Eisenacher and Christian Stephan.en_US
dc.rightsAttribution CC BY-NC-NDeng
dc.subjectData interpretationeng
dc.subjectOnline resourceeng
dc.subjectProtein functioneng
dc.titleIntroduction to opportunities and pitfalls in functional mass spectrometry based proteomicsen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2013 Elsevier B.V.
dc.source.journalBiochimica et Biophysica Acta - Proteins and Proteomics
dc.subject.nsiVDP::Medisinske fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk biokjemi: 726
dc.subject.nsiVDP::Midical sciences: 700::Basic medical, dental and veterinary sciences: 710::Medical biochemistry: 726

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