dc.contributor.author | Berge, Tone | en_US |
dc.contributor.author | Eriksson, Anna | en_US |
dc.contributor.author | Brorson, Ina Skaara | en_US |
dc.contributor.author | Høgestøl, Einar August | en_US |
dc.contributor.author | Berg-Hansen, Pål | en_US |
dc.contributor.author | Døskeland, Anne Marie Simonne | en_US |
dc.contributor.author | Mjaavatten, Olav | en_US |
dc.contributor.author | Bos, Steffan Daniel | en_US |
dc.contributor.author | Harbo, Hanne Flinstad | en_US |
dc.contributor.author | Berven, Frode | en_US |
dc.date.accessioned | 2020-06-08T13:05:57Z | |
dc.date.available | 2020-06-08T13:05:57Z | |
dc.date.issued | 2019-05-08 | |
dc.Published | Berge T, Eriksson A, Brorson IS, Høgestøl EA, Berg-Hansen PBH, Døskeland AP, Mjaavatten O, Bos SD, Harbo HFH, Berven F. Quantitative proteomic analyses of CD4+ and CD8+ T cells reveal differentially expressed proteins in multiple sclerosis patients and healthy controls . Clinical Proteomics. 2019;16:19 | eng |
dc.identifier.issn | 1542-6416 | |
dc.identifier.issn | 1559-0275 | |
dc.identifier.uri | https://hdl.handle.net/1956/22491 | |
dc.description.abstract | Background Multiple sclerosis (MS) is an autoimmune, neuroinflammatory disease, with an unclear etiology. However, T cells play a central role in the pathogenesis by crossing the blood–brain-barrier, leading to inflammation of the central nervous system and demyelination of the protective sheath surrounding the nerve fibers. MS has a complex inheritance pattern, and several studies indicate that gene interactions with environmental factors contribute to disease onset. Methods In the current study, we evaluated T cell dysregulation at the protein level using electrospray liquid chromatography–tandem mass spectrometry to get novel insights into immune-cell processes in MS. We have analyzed the proteomic profiles of CD4+ and CD8+ T cells purified from whole blood from 13 newly diagnosed, treatment-naive female patients with relapsing–remitting MS and 14 age- and sex-matched healthy controls. Results An overall higher protein abundance was observed in both CD4+ and CD8+ T cells from MS patients when compared to healthy controls. The differentially expressed proteins were enriched for T-cell specific activation pathways, especially CTLA4 and CD28 signaling in CD4+ T cells. When selectively analyzing proteins expressed from the genes most proximal to > 200 non-HLA MS susceptibility polymorphisms, we observed differential expression of eight proteins in T cells between MS patients and healthy controls, and there was a correlation between the genotype at three MS genetic risk loci and protein expressed from proximal genes. Conclusion Our study provides evidence for proteomic differences in T cells from relapsing–remitting MS patients compared to healthy controls and also identifies dysregulation of proteins encoded from MS susceptibility genes. | en_US |
dc.language.iso | eng | eng |
dc.publisher | BMC | eng |
dc.rights | Attribution CC BY | eng |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | eng |
dc.title | Quantitative proteomic analyses of CD4+ and CD8+ T cells reveal differentially expressed proteins in multiple sclerosis patients and healthy controls | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.date.updated | 2019-11-13T12:59:38Z | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2019 The Author(s) | |
dc.identifier.doi | https://doi.org/10.1186/s12014-019-9241-5 | |
dc.identifier.cristin | 1709560 | |
dc.source.journal | Clinical Proteomics | |
dc.relation.project | Norges forskningsråd: 240102 | |
dc.relation.project | Helse Sør-Øst RHF: 2017114 | |