Show simple item record

dc.contributor.authorBerge, Toneen_US
dc.contributor.authorEriksson, Annaen_US
dc.contributor.authorBrorson, Ina Skaaraen_US
dc.contributor.authorHøgestøl, Einar Augusten_US
dc.contributor.authorBerg-Hansen, Pålen_US
dc.contributor.authorDøskeland, Anne Marie Simonneen_US
dc.contributor.authorMjaavatten, Olaven_US
dc.contributor.authorBos, Steffan Danielen_US
dc.contributor.authorHarbo, Hanne Flinstaden_US
dc.contributor.authorBerven, Frodeen_US
dc.PublishedBerge T, Eriksson A, Brorson IS, Høgestøl EA, Berg-Hansen PBH, Døskeland AP, Mjaavatten O, Bos SD, Harbo HFH, Berven F. Quantitative proteomic analyses of CD4+ and CD8+ T cells reveal differentially expressed proteins in multiple sclerosis patients and healthy controls . Clinical Proteomics. 2019;16:19eng
dc.description.abstractBackground Multiple sclerosis (MS) is an autoimmune, neuroinflammatory disease, with an unclear etiology. However, T cells play a central role in the pathogenesis by crossing the blood–brain-barrier, leading to inflammation of the central nervous system and demyelination of the protective sheath surrounding the nerve fibers. MS has a complex inheritance pattern, and several studies indicate that gene interactions with environmental factors contribute to disease onset. Methods In the current study, we evaluated T cell dysregulation at the protein level using electrospray liquid chromatography–tandem mass spectrometry to get novel insights into immune-cell processes in MS. We have analyzed the proteomic profiles of CD4+ and CD8+ T cells purified from whole blood from 13 newly diagnosed, treatment-naive female patients with relapsing–remitting MS and 14 age- and sex-matched healthy controls. Results An overall higher protein abundance was observed in both CD4+ and CD8+ T cells from MS patients when compared to healthy controls. The differentially expressed proteins were enriched for T-cell specific activation pathways, especially CTLA4 and CD28 signaling in CD4+ T cells. When selectively analyzing proteins expressed from the genes most proximal to > 200 non-HLA MS susceptibility polymorphisms, we observed differential expression of eight proteins in T cells between MS patients and healthy controls, and there was a correlation between the genotype at three MS genetic risk loci and protein expressed from proximal genes. Conclusion Our study provides evidence for proteomic differences in T cells from relapsing–remitting MS patients compared to healthy controls and also identifies dysregulation of proteins encoded from MS susceptibility genes.en_US
dc.rightsAttribution CC BYeng
dc.titleQuantitative proteomic analyses of CD4+ and CD8+ T cells reveal differentially expressed proteins in multiple sclerosis patients and healthy controlsen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2019 The Author(s)
dc.source.journalClinical Proteomics
dc.relation.projectNorges forskningsråd: 240102
dc.relation.projectHelse Sør-Øst RHF: 2017114

Files in this item


This item appears in the following Collection(s)

Show simple item record

Attribution CC BY
Except where otherwise noted, this item's license is described as Attribution CC BY