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dc.contributor.authorBerge, Toneen_US
dc.contributor.authorEriksson, Annaen_US
dc.contributor.authorBrorson, Ina Skaaraen_US
dc.contributor.authorHøgestøl, Einar Augusten_US
dc.contributor.authorBerg-Hansen, Pålen_US
dc.contributor.authorDøskeland, Anne Marie Simonneen_US
dc.contributor.authorMjaavatten, Olaven_US
dc.contributor.authorBos, Steffan Danielen_US
dc.contributor.authorHarbo, Hanne Flinstaden_US
dc.contributor.authorBerven, Frodeen_US
dc.date.accessioned2020-06-08T13:05:57Z
dc.date.available2020-06-08T13:05:57Z
dc.date.issued2019-05-08
dc.PublishedBerge T, Eriksson A, Brorson IS, Høgestøl EA, Berg-Hansen PBH, Døskeland AP, Mjaavatten O, Bos SD, Harbo HFH, Berven F. Quantitative proteomic analyses of CD4+ and CD8+ T cells reveal differentially expressed proteins in multiple sclerosis patients and healthy controls . Clinical Proteomics. 2019;16:19eng
dc.identifier.issn1542-6416
dc.identifier.issn1559-0275
dc.identifier.urihttps://hdl.handle.net/1956/22491
dc.description.abstractBackground Multiple sclerosis (MS) is an autoimmune, neuroinflammatory disease, with an unclear etiology. However, T cells play a central role in the pathogenesis by crossing the blood–brain-barrier, leading to inflammation of the central nervous system and demyelination of the protective sheath surrounding the nerve fibers. MS has a complex inheritance pattern, and several studies indicate that gene interactions with environmental factors contribute to disease onset. Methods In the current study, we evaluated T cell dysregulation at the protein level using electrospray liquid chromatography–tandem mass spectrometry to get novel insights into immune-cell processes in MS. We have analyzed the proteomic profiles of CD4+ and CD8+ T cells purified from whole blood from 13 newly diagnosed, treatment-naive female patients with relapsing–remitting MS and 14 age- and sex-matched healthy controls. Results An overall higher protein abundance was observed in both CD4+ and CD8+ T cells from MS patients when compared to healthy controls. The differentially expressed proteins were enriched for T-cell specific activation pathways, especially CTLA4 and CD28 signaling in CD4+ T cells. When selectively analyzing proteins expressed from the genes most proximal to > 200 non-HLA MS susceptibility polymorphisms, we observed differential expression of eight proteins in T cells between MS patients and healthy controls, and there was a correlation between the genotype at three MS genetic risk loci and protein expressed from proximal genes. Conclusion Our study provides evidence for proteomic differences in T cells from relapsing–remitting MS patients compared to healthy controls and also identifies dysregulation of proteins encoded from MS susceptibility genes.en_US
dc.language.isoengeng
dc.publisherBMCeng
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/eng
dc.titleQuantitative proteomic analyses of CD4+ and CD8+ T cells reveal differentially expressed proteins in multiple sclerosis patients and healthy controlsen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2019-11-13T12:59:38Z
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2019 The Author(s)
dc.identifier.doihttps://doi.org/10.1186/s12014-019-9241-5
dc.identifier.cristin1709560
dc.source.journalClinical Proteomics
dc.relation.projectNorges forskningsråd: 240102
dc.relation.projectHelse Sør-Øst RHF: 2017114


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