dc.contributor.author | Ashrafi, Hanan | en_US |
dc.contributor.author | Siraji, Muntequa Ishtiaq | en_US |
dc.contributor.author | Showva, Nazmir Nur | en_US |
dc.contributor.author | Hossain, Md. Mozamme | en_US |
dc.contributor.author | Hossan, Tareq | en_US |
dc.contributor.author | Hasan, Md. Ashraful | en_US |
dc.contributor.author | Shohael, Abdullah Mohammad | en_US |
dc.contributor.author | Shawan, Mohammad Mahfuz Ali Khan | en_US |
dc.date.accessioned | 2020-06-08T14:39:07Z | |
dc.date.available | 2020-06-08T14:39:07Z | |
dc.date.issued | 2019-07-31 | |
dc.Published | Ashrafi H, Siraji MI, Showva, Hossain, Hossan, Hasan, Shohael, Shawan. Structure to function analysis with antigenic characterization of a hypothetical protein,HPAG1_0576 from Helicobacter pylori HPAG1. Bioinformation. 2019;15(7):456-466 | eng |
dc.identifier.issn | 0973-2063 | |
dc.identifier.issn | 0973-8894 | |
dc.identifier.uri | https://hdl.handle.net/1956/22500 | |
dc.description.abstract | Helicobacter pylori, a unique gastric pathogen causing chronic inflammation in the gastric mucosa with a possibility to develop gastric cancer has one-third of its proteins still uncharacterized. In this study, a hypothetical protein (HP) namely HPAG1_0576 from H. pylori HPAG1 was chosen for detailed computational analysis of its structural, functional and epitopic properties. The primary, secondary and 3D structure/model of the selected HP was constructed. Then refinement and structure validation were done, which indicated a good quality of the newly constructed model. ProFunc and STRING suggested that HPAG1_0576 shares 98% identity with a carcinogenic factor, TNF-α inducing protein (Tip-α) of H. pylori. IEDB immunoinformatics tool predicted VLMLQACTCPNTSQRNS from position 19-35 as most potential B-cell linear epitope and SFLKSKQL from position 5-12 as most potent conformational epitope. Alternatively, FALVRARGF and FLCGLGVLM were predicted as most immunogenic CD8+ and CD4+ T-cell epitopes respectively. At the same time findings of IFN epitope tool suggests that, HPAG1_0576 had a great potential to evoke interferon-gamma (IFN-γ) mediated immune response. However, this experiment is a primary approach for in silico vaccine designing from a HP, findings of this study will provide significant insights in further investigations and will assist in identifying new drug targets/vaccine candidates. | en_US |
dc.language.iso | eng | eng |
dc.publisher | Biomedical Informatics | eng |
dc.rights | Attribution CC BY | eng |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | eng |
dc.subject | H. pylori HPAG1 | eng |
dc.subject | hypothetical protein | eng |
dc.subject | B and T cell epitopes | eng |
dc.subject | drug target and vaccine candidates | eng |
dc.title | Structure to function analysis with antigenic characterization of a hypothetical protein, HPAG1_0576 from Helicobacter pylori HPAG1 | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.date.updated | 2020-01-30T08:34:22Z | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2019 Biomedical Informatics | |
dc.identifier.doi | https://doi.org/10.6026/97320630015456 | |
dc.identifier.cristin | 1785892 | |
dc.source.journal | Bioinformation | |