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dc.contributor.authorAshrafi, Hananen_US
dc.contributor.authorSiraji, Muntequa Ishtiaqen_US
dc.contributor.authorShowva, Nazmir Nuren_US
dc.contributor.authorHossain, Md. Mozammeen_US
dc.contributor.authorHossan, Tareqen_US
dc.contributor.authorHasan, Md. Ashrafulen_US
dc.contributor.authorShohael, Abdullah Mohammaden_US
dc.contributor.authorShawan, Mohammad Mahfuz Ali Khanen_US
dc.PublishedAshrafi H, Siraji MI, Showva, Hossain, Hossan, Hasan, Shohael, Shawan. Structure to function analysis with antigenic characterization of a hypothetical protein,HPAG1_0576 from Helicobacter pylori HPAG1. Bioinformation. 2019;15(7):456-466eng
dc.description.abstractHelicobacter pylori, a unique gastric pathogen causing chronic inflammation in the gastric mucosa with a possibility to develop gastric cancer has one-third of its proteins still uncharacterized. In this study, a hypothetical protein (HP) namely HPAG1_0576 from H. pylori HPAG1 was chosen for detailed computational analysis of its structural, functional and epitopic properties. The primary, secondary and 3D structure/model of the selected HP was constructed. Then refinement and structure validation were done, which indicated a good quality of the newly constructed model. ProFunc and STRING suggested that HPAG1_0576 shares 98% identity with a carcinogenic factor, TNF-α inducing protein (Tip-α) of H. pylori. IEDB immunoinformatics tool predicted VLMLQACTCPNTSQRNS from position 19-35 as most potential B-cell linear epitope and SFLKSKQL from position 5-12 as most potent conformational epitope. Alternatively, FALVRARGF and FLCGLGVLM were predicted as most immunogenic CD8+ and CD4+ T-cell epitopes respectively. At the same time findings of IFN epitope tool suggests that, HPAG1_0576 had a great potential to evoke interferon-gamma (IFN-γ) mediated immune response. However, this experiment is a primary approach for in silico vaccine designing from a HP, findings of this study will provide significant insights in further investigations and will assist in identifying new drug targets/vaccine candidates.en_US
dc.publisherBiomedical Informaticseng
dc.rightsAttribution CC BYeng
dc.subjectH. pylori HPAG1eng
dc.subjecthypothetical proteineng
dc.subjectB and T cell epitopeseng
dc.subjectdrug target and vaccine candidateseng
dc.titleStructure to function analysis with antigenic characterization of a hypothetical protein, HPAG1_0576 from Helicobacter pylori HPAG1en_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2019 Biomedical Informatics

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