Detecting single amino acids and small peptides by combining isobaric tags and peptidomics
Peer reviewed, Journal article
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Original versionEuropean Journal of Mass Spectrometry. 2019, 25 (6), 451–456. https://doi.org/10.1177/1469066719857006
Single amino acids and small endogenous peptides play important roles in maintaining a properly functioning organism. These molecules are however currently only routinely identified in targeted approaches. In a small proof-of-concept mass spectrometry experiment, we found that by combining isobaric tags and peptidomics, and by targeting singly charged molecules, we were able to identify a significant amount of single amino acids and small endogenous peptides using a basic mass-based identification approach. While there is still room for improvement, our simple test indicates that a limited amount of extra work when setting up the mass spectrometry experiment could potentially lead to a wealth of additional information.