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Detecting single amino acids and small peptides by combining isobaric tags and peptidomics

Burger, Bram; Lereim, Ragnhild Reehorst; Berven, Frode; Barsnes, Harald
Peer reviewed, Journal article
Accepted version
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https://hdl.handle.net/1956/22962
Utgivelsesdato
2019
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  • Department of Informatics [748]
Originalversjon
European Journal of Mass Spectrometry. 2019, 25 (6), 451–456.   https://doi.org/10.1177/1469066719857006
Sammendrag
Single amino acids and small endogenous peptides play important roles in maintaining a properly functioning organism. These molecules are however currently only routinely identified in targeted approaches. In a small proof-of-concept mass spectrometry experiment, we found that by combining isobaric tags and peptidomics, and by targeting singly charged molecules, we were able to identify a significant amount of single amino acids and small endogenous peptides using a basic mass-based identification approach. While there is still room for improvement, our simple test indicates that a limited amount of extra work when setting up the mass spectrometry experiment could potentially lead to a wealth of additional information.
Utgiver
SAGE
Tidsskrift
European Journal of Mass Spectrometry
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Copyright 2019 The Authors

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