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dc.contributor.authorHaslene-Hox, Hanneen_US
dc.contributor.authorOveland, Eysteinen_US
dc.contributor.authorBerg, Kaja Christine Graueen_US
dc.contributor.authorKolmannskog, Odden_US
dc.contributor.authorWoie, Kathrineen_US
dc.contributor.authorSalvesen, Helga Birgitteen_US
dc.contributor.authorTenstad, Olaven_US
dc.contributor.authorWiig, Helgeen_US
dc.description.abstractMajor efforts have been invested in the identification of cancer biomarkers in plasma, but the extraordinary dynamic range in protein composition, and the dilution of disease specific proteins make discovery in plasma challenging. Focus is shifting towards using proximal fluids for biomarker discovery, but methods to verify the isolated sample’s origin are missing. We therefore aimed to develop a technique to search for potential candidate proteins in the proximal proteome, i.e. in the tumor interstitial fluid, since the biomarkers are likely to be excreted or derive from the tumor microenvironment. Since tumor interstitial fluid is not readily accessible, we applied a centrifugation method developed in experimental animals and asked whether interstitial fluid from human tissue could be isolated, using ovarian carcinoma as a model. Exposure of extirpated tissue to 106 g enabled tumor fluid isolation. The fluid was verified as interstitial by an isolated fluid:plasma ratio not significantly different from 1.0 for both creatinine and Na+, two substances predominantly present in interstitial fluid. The isolated fluid had a colloid osmotic pressure 79% of that in plasma, suggesting that there was some sieving of proteins at the capillary wall. Using a proteomic approach we detected 769 proteins in the isolated interstitial fluid, sixfold higher than in patient plasma. We conclude that the isolated fluid represents undiluted interstitial fluid and thus a subproteome with high concentration of locally secreted proteins that may be detected in plasma for diagnostic, therapeutic and prognostic monitoring by targeted methods.en_US
dc.publisherPublic Library of Scienceeng
dc.relation.ispartof<a href="" target="blank">The microenvironment in human ovarian carcinoma - characterization through proteomic analysis of tissue interstitial fluid</a>eng
dc.rightsAttribution CC BYeng
dc.titleA New Method for Isolation of Interstitial Fluid from Human Solid Tumors Applied to Proteomic Analysis of Ovarian Carcinoma Tissueen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderCopyright 2011 Haslene-Hox et al.
dc.source.journalPLoS ONE
dc.subject.nsiVDP::Mathematics and natural science: 400::Basic biosciences: 470eng
dc.subject.nsiVDP::Medical disciplines: 700::Clinical medical disciplines: 750eng

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