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dc.contributor.authorSalma, Wahidaen_US
dc.contributor.authorZhou, Zhigangen_US
dc.contributor.authorWang, Wenwanen_US
dc.contributor.authorAskarian, Fatemehen_US
dc.contributor.authorKousha, Arminen_US
dc.contributor.authorEbrahimi, Maryam Tajabadien_US
dc.contributor.authorMyklebust, Reidaren_US
dc.contributor.authorRingø, Einaren_US
dc.date.accessioned2013-05-02T09:24:12Z
dc.date.available2013-05-02T09:24:12Z
dc.date.issued2011-04-04eng
dc.PublishedAquaculture 314(1–4): 24–33eng
dc.identifier.issn0044-8486
dc.identifier.urihttps://hdl.handle.net/1956/6551
dc.description.abstractIn the present study the intestinal sac method (ex vivo) was used to evaluate the interactions between lactic acid bacteria and staphylococci in the gastrointestinal (GI) tract of beluga (Huso huso). The distal intestine (DI) of beluga was exposed ex vivo to Staphylococcus aureus, Leuconostoc mesenteroides and Lactobacillus plantarum. Histological changes following bacterial exposure were assessed by light and electron microscopy. Control samples and samples exposed only to Leu. mesenteroides and a combination of Leu. mesenteroides and Staph. aureus, had a similar appearance to intact intestinal mucosal epithelium, with no signs of cellular damage. However, exposure of the DI to Staph. aureus and L. plantarum resulted in damaged epithelial cells and disorganized microvilli. Furthermore, 16S rDNA PCR denaturing gradient gel electrophoresis (PCR-DGGE) was used to investigate the adherent microbiota of distal beluga intestine. Several bacterial species were identified by DGGE in the present study that have not previously been identified in beluga.en_US
dc.language.isoengeng
dc.publisherElseviereng
dc.rights.urihttp://www.elsevier.com/authors/author-rights-and-responsibilitieseng
dc.subjectBelugaeng
dc.subjectIntestineeng
dc.subjectBacteriaeng
dc.subjectLight and electron microscopyeng
dc.subjectDGGEeng
dc.titleHistological and bacteriological changes in intestine of beluga (Huso huso) following ex vivo exposure to bacterial strainsen_US
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionacceptedVersionen_US
dc.rights.holderCopyright 2011 Elsevier
dc.identifier.doihttps://doi.org/10.1016/j.aquaculture.2011.01.047
dc.identifier.cristin840082
dc.source.journalAquaculture
dc.source.40314
dc.source.141-4
dc.source.pagenumber24-33


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