Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors

Oudin, Anaïs; Baus, Virginie; Barthelemy, Vanessa; Fabian, Carina; Klein, Eliane; Dieterle, Monika; Wantz, May; Hau, Ann-Christin; Dording, Claire; Bernard, Amandine; Michelucci, Alessandro; Yabo, Yahaya A.; Kanli, Georgia; Keunen, Olivier; Bjerkvig, Rolf; Niclou, Simone Pierrette; Golebiewska, Anna
Journal article, Peer reviewed
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https://hdl.handle.net/11250/2991017
Date
2021
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STAR Protocols. 2021, 2 (2), 100534.   10.1016/j.xpro.2021.100534
Abstract
Tumor organoids and patient-derived orthotopic xenografts (PDOXs) are some of the most valuable pre-clinical tools in cancer research. In this protocol, we describe efficient derivation of organoids and PDOX models from glioma patient tumors. We provide detailed steps for organoid culture, intracranial implantation, and detection of tumors in the brain. We further present technical adjustments for standardized functional assays and drug testing.
Publisher
Cell Press
Journal
STAR Protocols
Copyright
Copyright 2021 The Author(s)
Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal