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dc.contributor.authorMinsaas, Lauraen_US
dc.contributor.authorPlanagumà, Jesúsen_US
dc.contributor.authorMadziva, Michaelen_US
dc.contributor.authorKrakstad, Beate F.en_US
dc.contributor.authorMasià-Balagué, Míriamen_US
dc.contributor.authorKatz, Arieh A.en_US
dc.contributor.authorAragay, Anna M.en_US
dc.PublishedPLoS ONE 5(8): e12212en
dc.description.abstractThe chemokine (C-C motif) receptor 2B (CCR2B) is one of the two isoforms of the receptor for monocyte chemoattractant protein-1 (CCL2), the major chemoattractant for monocytes, involved in an array of chronic inflammatory diseases. Employing the yeast two-hybrid system, we identified the actin-binding protein filamin A (FLNa) as a protein that associates with the carboxyl-terminal tail of CCR2B. Co-immunoprecipitation experiments and in vitro pull down assays demonstrated that FLNa binds constitutively to CCR2B. The colocalization of endogenous CCR2B and filamin A was detected at the surface and in internalized vesicles of THP-1 cells. In addition, CCR2B and FLNa were colocalized in lamellipodia structures of CCR2B-expressing A7 cells. Expression of the receptor in filamin-deficient M2 cells together with siRNA experiments knocking down FLNa in HEK293 cells, demonstrated that lack of FLNa delays the internalization of the receptor. Furthermore, depletion of FLNa in THP-1 monocytes by RNA interference reduced the migration of cells in response to MCP-1. Therefore, FLNa emerges as an important protein for controlling the internalization and spatial localization of the CCR2B receptor in different dynamic membrane structures.en_US
dc.publisherPublic Library of Scienceeng
dc.rightsAttribution CC BYeng
dc.titleFilamin A binds to CCR2B and regulates its internalizationen_US
dc.typePeer reviewed
dc.typeJournal article
dc.rights.holderMinsaas et al.
dc.rights.holderCopyright 2010 Minsaas et al.
dc.subject.nsiVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710nob

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