dc.contributor.author | Minsaas, Laura | en_US |
dc.contributor.author | Planagumà, Jesús | en_US |
dc.contributor.author | Madziva, Michael | en_US |
dc.contributor.author | Krakstad, Beate F. | en_US |
dc.contributor.author | Masià-Balagué, Míriam | en_US |
dc.contributor.author | Katz, Arieh A. | en_US |
dc.contributor.author | Aragay, Anna M. | en_US |
dc.date.accessioned | 2010-12-14T08:52:33Z | |
dc.date.available | 2010-12-14T08:52:33Z | |
dc.date.issued | 2010-08-17 | eng |
dc.Published | PLoS ONE 5(8): e12212 | en |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | https://hdl.handle.net/1956/4344 | |
dc.description.abstract | The chemokine (C-C motif) receptor 2B (CCR2B) is one of the two isoforms of the receptor for monocyte chemoattractant protein-1 (CCL2), the major chemoattractant for monocytes, involved in an array of chronic inflammatory diseases. Employing the yeast two-hybrid system, we identified the actin-binding protein filamin A (FLNa) as a protein that associates with the carboxyl-terminal tail of CCR2B. Co-immunoprecipitation experiments and in vitro pull down assays demonstrated that FLNa binds constitutively to CCR2B. The colocalization of endogenous CCR2B and filamin A was detected at the surface and in internalized vesicles of THP-1 cells. In addition, CCR2B and FLNa were colocalized in lamellipodia structures of CCR2B-expressing A7 cells. Expression of the receptor in filamin-deficient M2 cells together with siRNA experiments knocking down FLNa in HEK293 cells, demonstrated that lack of FLNa delays the internalization of the receptor. Furthermore, depletion of FLNa in THP-1 monocytes by RNA interference reduced the migration of cells in response to MCP-1. Therefore, FLNa emerges as an important protein for controlling the internalization and spatial localization of the CCR2B receptor in different dynamic membrane structures. | en_US |
dc.language.iso | eng | eng |
dc.publisher | Public Library of Science | eng |
dc.rights | Attribution CC BY | eng |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/ | eng |
dc.title | Filamin A binds to CCR2B and regulates its internalization | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Minsaas et al. | |
dc.rights.holder | Copyright 2010 Minsaas et al. | |
dc.identifier.doi | https://doi.org/10.1371/journal.pone.0012212 | |
dc.identifier.cristin | 347116 | |
dc.subject.nsi | VDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710 | nob |