Browsing Department of Biomedicine by Title
Now showing items 499-518 of 710
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PeptideShaker Online: A User-Friendly Web-Based Framework for the Identification of Mass Spectrometry-Based Proteomics Data
(Journal article; Peer reviewed, 2021)Mass spectrometry-based proteomics is a high-throughput technology generating ever-larger amounts of data per project. However, storing, processing, and interpreting these data can be a challenge. A key element in simplifying ... -
The Peripheral Binding of 14-3-3γ to Membranes Involves Isoform-Specific Histidine Residues
(Peer reviewed; Journal article, 2012-11-26)Mammalian 14-3-3 protein scaffolds include seven conserved isoforms that bind numerous phosphorylated protein partners and regulate many cellular processes. Some 14-3-3-isoforms, notably γ, have elevated affinity for ... -
Personality traits, risk factors and comorbidities in Attention-deficit/hyperactivity disorder
(Doctoral thesis, 2020-06-19)Background: Attention-deficit/hyperactivity disorder (ADHD) comprises cognitive and behavioural traits present from childhood. During lifespan, people with ADHD are more prone to develop psychiatric- and somatic comorbidities ... -
Personalized Medicine to Improve Treatment of Dopa-Responsive Dystonia—A Focus on Tyrosine Hydroxylase Deficiency
(Journal article; Peer reviewed, 2021)Dopa-responsive dystonia (DRD) is a rare movement disorder associated with defective dopamine synthesis. This impairment may be due to the fact of a deficiency in GTP cyclohydrolase I (GTPCHI, GCH1 gene), sepiapterin ... -
PFN2 and NAA80 cooperate to efficiently acetylate the N-terminus of actin
(Journal article; Peer reviewed, 2020)The actin cytoskeleton is of profound importance to cell shape, division, and intracellular force generation. Profilins bind to globular (G-)actin and regulate actin filament formation. Although profilins are well-established ... -
Pharmacokinetics and pharmacodynamics of t-cell bispecifics in the tumour interstitial fluid
(Journal article; Peer reviewed, 2021)The goal of this study is to investigate the pharmacokinetics in plasma and tumour interstitial fluid of two T-cell bispecifics (TCBs) with different binding affinities to the tumour target and to assess the subsequent ... -
A Pharmacological Chaperone Therapy for Acute Intermittent Porphyria
(Peer reviewed; Journal article, 2019-12-03)Mutations in hydroxymethylbilane synthase (HMBS) cause acute intermittent porphyria (AIP), an autosomal dominant disease where typically only one HMBS allele is mutated. In AIP, the accumulation of porphyrin precursors ... -
Phenylalanine Hydroxylase from Legionella pneumophila Is a Thermostable Enzyme with a Major Functional Role in Pyomelanin Synthesis
(Peer reviewed; Journal article, 2012-09-26)Background: Legionella pneumophila is a pathogenic bacterium that can cause Legionnaires’ disease and other non-pneumonic infections in humans. This bacterium produces a pyomelanin pigment, a potential virulence factor ... -
Phosphatidylserine receptors enhance SARS-CoV-2 infection
(Journal article; Peer reviewed, 2021)Phosphatidylserine (PS) receptors enhance infection of many enveloped viruses through virion-associated PS binding that is termed apoptotic mimicry. Here we show that this broadly shared uptake mechanism is utilized by ... -
Phosphoglycerate Mutase 1 Activates DNA Damage Repair via Regulation of WIP1 Activity
(Journal article; Peer reviewed, 2020)The metabolic enzyme phosphoglycerate mutase 1 (PGAM1) is overexpressed in several types of cancer, suggesting an additional function beyond its established role in the glycolytic pathway. We here report that PGAM1 is ... -
Phospholipase D in human blood platelets
(Doctoral thesis, 2008-05-30)We have studied phospholipase D (PLD) in human blood platelets. This enzyme hydrolyzes phosphatidylcholine to phosphatidic acid (PA) and choline, where PA is considered to be the main effector of PLDs function in cells. ... -
Phosphorylation at serine 31 targets tyrosine hydroxylase tovesicles for transport along microtubules
(Peer reviewed; Journal article, 2017)Tyrosine hydroxylase (TH) catalyzes the conversion of L-tyrosine into L-DOPA, which is the rate-limiting step in the synthesis of catecholamines, such as dopamine, in dopaminergergic neurons. Low dopamine levels and death ... -
PKM2 Interacts With the Cdk1-CyclinB Complex to Facilitate Cell Cycle Progression in Gliomas
(Journal article; Peer reviewed, 2022)PKM2 is a phosphotyrosine-binding glycolytic enzyme upregulated in many cancers, including glioma, and contributes to tumor growth by regulating cell cycle progression. We noted, however, that in multiple glioma cell lines, ... -
PKU mutation p.G46S prevents the stereospecific binding of l-phenylalanine to the dimer of human phenylalanine hydroxylase regulatory domain
(Peer reviewed; Journal article, 2017-02)Mammalian phenylalanine hydroxylase (PAH) has a potential allosteric regulatory binding site for l‐phenylalanine (l‐Phe), in addition to its catalytic site. This arrangement is supported by a crystal structure of a homodimeric ... -
PMEPA1 isoform a drives progression of glioblastoma by promoting protein degradation of the Hippo pathway kinase LATS1
(Peer reviewed; Journal article, 2019)The Hippo signaling pathway controls organ development and is also known, in cancer, to have a tumor suppressing role. Within the Hippo pathway, we here demonstrate, in human gliomas, a functional interaction of a transmembrane ... -
Post-translational modifications of Annexin A2 are linked to its association with perinuclear nonpolysomal mRNP complexes
(Peer reviewed; Journal article, 2017-02)Various post-translational modifications (PTMs) regulate the localisation and function of the multifunctional protein Annexin A2 (AnxA2). In addition to its various tasks as a cytoskeletal- and membrane-associated protein, ... -
Post-translational modifications of integrin ligands as pathogenic mechanisms in disease
(Peer reviewed; Journal article, 2014-11)Protein post-translational modifications like glycation, carbamylation and citrullination increase the functional diversity of the proteome but in disease situations might do more harm than good. Post-translational ... -
Potassium channels in behavioral brain disorders. Molecular mechanisms and therapeutic potential: A narrative review
(Journal article; Peer reviewed, 2023)Potassium channels (K+-channels) selectively control the passive flow of potassium ions across biological membranes and thereby also regulate membrane excitability. Genetic variants affecting many of the human K+-channels ... -
POU3f2 in human gliomas - Expression pattern and functional role
(Master thesis, 2014-06-02)Gliomas are tumors of the central nervous system (CNS). They are responsible for about 28% of all central nervous system tumors and for 80% of all malignant brain tumors. The most commonly used classification and grading ... -
Practical considerations for omics experiments in biomedical sciences
(Peer reviewed; Journal article, 2016)Modern analytical techniques provide an unprecedented insight to biomedical samples, allowing an in depth characterization of cells or body fluids, to the level of genes, transcripts, peptides, proteins, metabolites, or ...