Browsing Department of Biomedicine by Title
Now showing items 98-117 of 512
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Dactolisib (NVP-BEZ235) toxicity in murine brain tumour models
(Peer reviewed; Journal article, 2016-08-19)Background: Glioblastomas (GBMs) are highly malignant brain tumours with a poor prognosis, and current cytotoxic regimens provide only a limited survival benefit. The PI3K/Akt/mTOR pathway has been an attractive target for ... -
Deciphering the complex role of thrombospondin-1 in glioblastoma development
(Peer reviewed; Journal article, 2019-03-08)We undertook a systematic study focused on the matricellular protein Thrombospondin-1 (THBS1) to uncover molecular mechanisms underlying the role of THBS1 in glioblastoma (GBM) development. THBS1 was found to be increased ... -
Decoding cancer's camouflage: epithelialmesenchymal plasticity in resistance to immune checkpoint blockade
(Journal article, 2020)Epithelial-mesenchymal plasticity (EMP) of cancer cells contributes to cancer cell heterogeneity, and it is well established that EMP is a critical determinant of acquired resistance to cancer treatment modalities including ... -
Degradation of Extracellular NAD+ Intermediates in Cultures of Human HEK293 Cells
(Peer reviewed; Journal article, 2019-11-29)Nicotinamide adenine dinucleotide (NAD) is an essential redox carrier, whereas its degradation is a key element of important signaling pathways. Human cells replenish their NAD contents through NAD biosynthesis from ... -
Dendritic cells in Sjögren's syndrome-functional analyses
(Master thesis, 2009-06-02)Sjögren's syndrome (SS) is an autoimmune disease of unknown etiology. It is characterized by chronic inflammation of the exocrine glands which leads to dryness of the mouth and eyes. Dendritic cells (DC) are the most potent ... -
Dendritic cells loaded with tumor antigens and a dual immunostimulatory and anti-IL-10 specific siRNA can prime T lymphocytes against leukemic cells
(Peer reviewed; Journal article, 2009-12)Vaccines using dendritic cells (DCs) harboring leukemic antigens to stimulate T cells is a possible treatment of acute myeloid leukemia (AML). Limitations of breaking tolerance to leukemic cells and lack of specific ... -
DeNovoGUI: an open source graphical user interface for de novo sequencing of tandem mass spectra
(Peer reviewed; Journal article, 2014)De novo sequencing is a popular technique in proteomics for identifying peptides from tandem mass spectra without having to rely on a protein sequence database. Despite the strong potential of de novo sequencing algorithms, ... -
Design of tumor-specific immunotherapies using dendritic cells - effect of bromelain on dendritic cell maturation
(Master thesis, 2009-06-02)Immunotherapy using dendritic cells (DC) has shown promising results in clinical trials, but few relevant successes are recorded. Therefore, the choice of an appropriate DC population is critical for the outcome of this ... -
Design of tumour-specific immunotherapies using dendritic cells – analyses of IL15-DC
(Master thesis, 2009-05-31)Immunotherapy of malignancies aims at activating the patient’s own immune system to fight the tumour affecting the patient. Even though the use of dendritic cells (DC) has shown promising results, the DC vaccination strategy ... -
Determinants of ligand binding and catalytic activity in the myelin enzyme 2′,3′-cyclic nucleotide 3′-phosphodiesterase
(Peer reviewed; Journal article, 2015-11-13)2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) is an enzyme highly abundant in the central nervous system myelin of terrestrial vertebrates. The catalytic domain of CNPase belongs to the 2H phosphoesterase superfamily ... -
Developing Neurons Form Transient Nanotubes Facilitating Electrical Coupling and Calcium Signaling with Distant Astrocytes
(Peer reviewed; Journal article, 2012-10-11)Despite the well-documented cooperation between neurons and astrocytes little is known as to how these interactions are initiated. We show here by differential interference contrast microscopy that immature hippocampal ... -
Development of an RNA Interference Tool, Characterization of Its Target, and an Ecological Test of Caste Differentiation in the Eusocial Wasp Polistes
(Peer reviewed; Journal article, 2011-11-01)Recent advancements in genomics provide new tools for evolutionary ecological research. The paper wasp genus Polistes is a model for social insect evolution and behavioral ecology. We developed RNA interference (RNAi)-mediated ... -
Development of robust targeted proteomics assays for cerebrospinal fluid biomarkers in multiple sclerosis
(Journal article; Peer reviewed, 2020)Background: Verification of cerebrospinal fluid (CSF) biomarkers for multiple sclerosis and other neurological diseases is a major challenge due to a large number of candidates, limited sample material availability, disease ... -
Diabetic hyperglycemia reduces Ca2+ permeability of extrasynaptic AMPA receptors in AII amacrine cells
(Peer reviewed; Journal article, 2015-09)There is increasing evidence that diabetic retinopathy is a primary neuropathological disorder that precedes the microvascular pathology associated with later stages of the disease. Recently, we found evidence for altered ... -
Diazoxide protects against doxorubicin-induced cardiotoxicity in the rat
(Peer reviewed; Journal article, 2014-05-27)Aim: Chemotherapy with doxorubicin is limited by cardiotoxicity. Free radical generation and mitochondrial dysfunction are thought to contribute to doxorubicin-induced cardiac failure. In this study we wanted to investigate ... -
Dietary linoleic acid elevates the endocannabinoids 2-AG and anandamide and promotes weight gain in mice fed a low fat diet
(Peer reviewed; Journal article, 2014-01)Dietary intake of linoleic acid (LNA, 18:2n-6) has increased dramatically during the 20th century and is associated with greater prevalence of obesity. The endocannabinoid system is involved in regulation of energy balance ... -
Differential contribution of gap junctions to the membrane properties of ON- and OFF-bipolar cells of the rat retina
(Journal article; Peer reviewed, 2020)Gap junctions are ubiquitous within the retina, but in general, it remains to be determined whether gap junction coupling between specific cell types is sufficiently strong to mediate functionally relevant coupling via ... -
Differential expression patterns of conserved miRNAs and isomiRs during Atlantic halibut development
(Peer reviewed; Journal article, 2012-01-10)Background: MicroRNAs (miRNAs) play a major role in animal ontogenesis. Size variants of miRNAs, isomiRs, are observed along with the main miRNA types, but their origin and possible biological role are uncovered yet. ... -
Direct Binding of the Flexible C-Terminal Segment of Periaxin to β4 Integrin Suggests a Molecular Basis for CMT4F
(Peer reviewed; Journal article, 2019-04-09)The process of myelination in the nervous system requires a coordinated formation of both transient and stable supramolecular complexes. Myelin-specific proteins play key roles in these assemblies, which may link membranes ... -
Discovery and biological characterization of a novel scaffold for potent inhibitors of peripheral serotonin synthesis
(Journal article; Peer reviewed, 2020)Aim: Tryptophan hydroxylase 1 (TPH1) catalyzes serotonin synthesis in peripheral tissues. Selective TPH1 inhibitors may be useful for treating disorders related to serotonin dysregulation. Results & methodology: Screening ...